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Sample GSM34074 Query DataSets for GSM34074
Status Public on Jul 01, 2005
Title TGCT cell line 2102Ep/clone 2A6, treated 3 days with retinoic acid, undifferentiated
Sample type RNA
 
Channel 1
Source name TGCT cell line 2102Ep/clone 2A6, treated 3 days with retinoic acid, undifferentiated
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name Universal Human Reference RNA (Stratagene)
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description Testicular germ cell tumor (embryonal carcinoma) cell line 2102Ep (clone 2A6). Embryonal carcinoma cell lines are generally pluripotent and share features with embryonic stem cells. However, the 2102Ep cell line is relatively nullipotent. Time point 3 days of culturing with retinoic acid (still undifferentiated). Maintained at high cell density (>5 000 000 cells per 75 cm2 tissue culture flask) in Dulbecco Modified Eagles Medium (DMEM high glucose formulation; GIBCO LifeTechnologies, UK) supplemented with 10% fetal calf serum.
Keywords = cell line
Keywords = differentiation
Keywords = embryonal carcinoma
Keywords = embryonic stem cell
Keywords = nullipotency
Keywords = nullipotent
Keywords = pluripotency
Keywords = pluripotent
Keywords = retinoic acid
Keywords = seminoma
Keywords = teratocarcinoma
Keywords = testicular germ cell tumor
Keywords = testicular neoplasm
Keywords = testis
Keywords = totipotency
Keywords = undifferentiated
 
Submission date Oct 29, 2004
Last update date Mar 09, 2006
Contact name Rolf I Skotheim
E-mail(s) rolf.i.skotheim@rr-research.no
Phone +47 2293 4000
Fax +47 2293 5767
URL http://www.radium.no/cancerprevention
Organization name Rikshospitalet-Radiumhospitalet Medical Center, Institute for Cancer Research
Department Department of Cancer Prevention
Street address
City Oslo
ZIP/Postal code N-0310
Country Norway
 
Platform ID GPL885
Series (1)
GSE1818 Testicular germ cell tumors and their histological subgroups

Data table header descriptions
ID_REF
FLAG Boolean for measurement quality. 1=Feature with inadequate measurements, 0=Feature with good quality measurements
VALUE same as UNF_VALUE but with flagged values removed
Log2_NormalTestisCenteredRatio For features with FLAG=0: Log2(SampleRATIO/MedianRATIO of the three Normal testis samples); gives an indication of up- and downregulation in the sample relative to the mRNA level in normal testis
RATIO For features with FLAG=0: CH1_ProcessedSignal / CH2_ProcessedSignal
PositionX X-coordinate, pixel position, of feature centroid on the original scan (tif-image)
PositionY Y-coordinate, pixel position, of feature centroid on the original scan (tif-image)
CH1_SurrogateUsed The CH1 surrogate value used
CH2_SurrogateUsed The CH2 surrogate value used
CH1_ProcessedSignal The propagated CH1 feature signal, used further for computation of ratios and log2ratios
CH2_ProcessedSignal The propagated CH2 feature signal, used further for computation of ratios and log2ratios
CH1_ProcessedSigError Standard error of propagated CH1 feature signal
CH2_ProcessedSigError Standard error of propagated CH2 feature signal
CH1_AREA Total number of pixels used to compute CH1 feature statistics
CH2_AREA Total number of pixels used to compute CH2 feature statistics
CH1_MEAN Raw mean signal of feature in CH1
CH2_MEAN Raw mean signal of feature in CH2
CH1_MEDIAN Raw median signal of feature in CH1
CH2_MEDIAN Raw median signal of feature in CH2
CH1_SD Standard deviation of all inlier pixels per CH1 feature
CH2_SD Standard deviation of all inlier pixels per CH2 feature
CH1_BKD_AREA Total number of pixels used to compute local CH1 background statistics per spot
CH2_BKD_AREA Total number of pixels used to compute local CH2 background statistics per spot
CH1_BKD_MEAN Mean local background CH1 signal (local to corresponding feature)
CH2_BKD_MEAN Mean local background CH2 signal (local to corresponding feature)
CH1_BKD_MEDIAN Median local background CH1 signal (local to corresponding feature)
CH2_BKD_MEDIAN Median local background CH2 signal (local to corresponding feature)
CH1_BKD_SD Standard deviation of all inlier pixels per local CH1 background of each feature
CH2_BKD_SD Standard deviation of all inlier pixels per local CH2 background of each feature
CH1_NumSatPix Total number of CH1 saturated pixels per feature
CH2_NumSatPix Total number of CH2 saturated pixels per feature
CH1_IsSaturated Boolean flag indicating if a feature is saturated for CH1 or not. 1=Saturated (>50% of the pixels above the saturation threshold), 0=NotSaturated
CH2_IsSaturated Boolean flag indicating if a feature is saturated for CH2 or not. 1=Saturated (>50% of the pixels above the saturation threshold), 0=NotSaturated
CH1_IsFeatNonUnifOL Boolean flag indicating if a feature is a NonUniformity Outlier or not for CH1. 1=The feature is non-uniform in that the pixel noise of feature exceeds a threshold established for a "uniform" feature, 0=Uniform feature
CH2_IsFeatNonUnifOL Boolean flag indicating if a feature is a NonUniformity Outlier or not for CH2. 1=The feature is non-uniform in that the pixel noise of feature exceeds a threshold established for a "uniform" feature, 0=Uniform feature
CH1_IsBGNonUnifOL Boolean flag indicating if a feature is a Background NonUniformity Outlier or not for CH1. 1=The background is non-uniform in that the pixel noise of feature background exceeds a threshold established for a "uniform" background, 0=Uniform background
CH2_IsBGNonUnifOL Boolean flag indicating if a feature is a Background NonUniformity Outlier or not for CH2. 1=The background is non-uniform in that the pixel noise of feature background exceeds a threshold established for a "uniform" background, 0=Uniform background
CH1_IsFeatPopnOL Boolean flag indicating if a feature is a Population Outlier or not for CH1. Probes with replicate features on the microarray were examined using population statistics. 1=IsPopulationOutlier, 0=IsNotPopulationOutlier
CH2_IsFeatPopnOL Boolean flag indicating if a feature is a Population Outlier or not for CH2. Probes with replicate features on the microarray were examined using population statistics. 1=IsPopulationOutlier, 0=IsNotPopulationOutlier
CH1_BGSubSignal The net CH1 signal following the subtraction of the CH1 background from the raw mean CH1 signal
CH2_BGSubSignal The net CH2 signal following the subtraction of the CH2 background from the raw mean CH2 signal
CH1_BGSubSigError Propagated standard error as computed on net CH1 background subtracted signal
CH2_BGSubSigError Propagated standard error as computed on net CH2 background subtracted signal
CH1_IsWellAboveBG Boolean flag indicating if CH1 for a feature is WellAbove Background or not. 1=WellAboveBackground, 0=NotWellAboveBackground
CH2_IsWellAboveBG Boolean flag indicating if CH2 for a feature is WellAbove Background or not. 1=WellAboveBackground, 0=NotWellAboveBackground
IsNormalization A boolean flag which indicates if a feature is used to measure dye bias. 1=Feature used, 2=Feature not used.
CH1_DyeNormSignal The CH1 dye normalized signal
CH2_DyeNormSignal The CH2 dye normalized signal
CH1_DyeNormError The standard error associated with the CH1 dye normalized signal
CH2_DyeNormError The standard error associated with the CH2 dye normalized signal
DyeNormCorrelation Dye normalized CH1 and CH2 pixel correlation
UNF_VALUE Expression level (mRNA abundance) relative to the common universal human reference RNA. Log2(CH1_ProcessedSignal/CH2_Processed signal)

Data table
ID_REF FLAG VALUE Log2_NormalTestisCenteredRatio RATIO PositionX PositionY CH1_SurrogateUsed CH2_SurrogateUsed CH1_ProcessedSignal CH2_ProcessedSignal CH1_ProcessedSigError CH2_ProcessedSigError CH1_AREA CH2_AREA CH1_MEAN CH2_MEAN CH1_MEDIAN CH2_MEDIAN CH1_SD CH2_SD CH1_BKD_AREA CH2_BKD_AREA CH1_BKD_MEAN CH2_BKD_MEAN CH1_BKD_MEDIAN CH2_BKD_MEDIAN CH1_BKD_SD CH2_BKD_SD CH1_NumSatPix CH2_NumSatPix CH1_IsSaturated CH2_IsSaturated CH1_IsFeatNonUnifOL CH2_IsFeatNonUnifOL CH1_IsBGNonUnifOL CH2_IsBGNonUnifOL CH1_IsFeatPopnOL CH2_IsFeatPopnOL CH1_BGSubSignal CH2_BGSubSignal CH1_BGSubSigError CH2_BGSubSigError CH1_IsWellAboveBG CH2_IsWellAboveBG IsNormalization CH1_DyeNormSignal CH2_DyeNormSignal CH1_DyeNormError CH2_DyeNormError DyeNormCorrelation UNF_VALUE
1 0 0.703779171 -1.85041786 1.628765797 43.4606 39.2214 0 0 597.358 366.755 7.13899 5.2798 55 55 740.509 442.909 741 442 51.5644 39.2349 251 251 60.0478 74.1633 59 73 7.45048 10.7149 0 0 0 0 0 0 0 0 0 0 583.119 370.484 6.96882 5.33349 1 1 0 597.358 366.755 7.13899 5.2798 0.254565 0.703779171
2 1 66.1242 36.5033 8.01199 0 8.01199 12.9816 1.56193 1.20064 51 51 160.588 85.3529 161 86 8.63291 9.45267 285 285 57.9298 71.3333 57 70 6.50855 10.2976 0 0 0 0 0 0 0 0 0 0 5.31594 15.758 1.26884 1.45743 0 0 0 6.5439 12.9816 1.56193 1.20064 -0.170099 -0.696235683
3 0 0.03539888 -0.472642941 1.024840134 86.1554 37.9874 0 0 208.185 203.139 3.37668 4.85677 60 60 356.7 276.367 357.5 267 25.2595 37.9308 268 268 57.0373 71.9851 56.5 71 6.00488 9.06447 0 0 0 0 0 0 0 0 0 0 202.32 206.12 3.28156 4.92805 1 1 1 208.185 203.139 3.37668 4.85677 0.304044 0.03539888
4 0 -0.087700591 -0.914175949 0.941021382 107.915 37.8745 0 0 50646.9 53821.2 351.856 455.078 59 59 51666.8 52268.3 51420 52039 2748.44 3389.48 254 254 64.5945 81.5669 64 79 8.69901 13.606 0 0 0 0 0 0 0 0 0 0 51504.8 52188.5 357.817 441.273 1 1 1 50646.9 53821.2 351.856 455.078 0.967892 -0.087700591
5 0 -0.093371722 -0.372599178 0.937329555 129.395 39.1559 0 0 1051.74 1122.06 15.7395 17.0321 57 57 1205.39 1179.37 1212 1153 118.503 127.023 273 273 58.7216 72.0989 58 71 7.81468 9.23395 0 0 0 0 0 0 0 0 0 0 1049.32 1109.01 15.7033 16.8339 1 1 1 1051.74 1122.06 15.7395 17.0321 0.75896 -0.093371722
7 0 0.929746038 -2.109643527 1.904940634 171.521 37.4317 0 0 310.612 163.056 4.00272 4.27601 60 60 456.167 233.617 457.5 234 30.0193 33.3716 276 276 56.9312 69.9239 57 70 5.64839 8.46799 0 0 0 0 0 0 0 0 0 0 301.893 165.431 3.89036 4.3383 1 1 0 310.612 163.056 4.00272 4.27601 0.28291 0.929746038
8 0 2.60515997 2.95316941 6.084589654 193.118 37.2524 0 0 13133.1 2158.42 109.251 18.0995 56 56 13259.4 2204.14 13106 2209 815.557 133.809 266 266 61.0414 72.2744 59.5 71 7.87318 9.99716 0 0 0 0 0 0 0 0 0 0 13101 2133.61 108.984 17.8914 1 1 1 13133.1 2158.42 109.251 18.0995 0.650549 2.60515997
9 0 -0.569604419 -0.935762391 0.673801517 213.091 39.193 0 0 683.228 1013.99 8.38177 12.1412 62 62 841.339 1082.66 826.5 1078.5 65.2096 94.9037 242 242 66.4008 76.1983 64 75 11.1646 10.5403 0 0 0 0 0 0 0 0 0 0 677.595 1008.2 8.31267 12.0718 1 1 1 683.228 1013.99 8.38177 12.1412 0.648334 -0.569604419
11 0 0.017692405 -0.510869999 1.012338945 255.519 39.5315 0 0 456.576 451.011 6.88171 6.49093 56 56 599.911 524.625 597 525 50.174 48.8964 258 258 56.9806 70.6589 57 70 6.26053 9.08284 0 0 0 0 0 0 0 0 0 0 445.588 455.705 6.7161 6.55848 1 1 1 456.576 451.011 6.88171 6.49093 0.407085 0.017692405
13 0 -0.025372988 0.133272139 0.982566537 299.303 38.5424 0 0 353.045 359.309 5.8042 6.07985 59 59 498.542 434.797 501 429 43.2839 46.9728 233 233 57.2103 72.897 56 73 7.29983 10.3897 0 0 0 0 0 0 0 0 0 0 343.99 363.638 5.65534 6.1531 1 1 1 353.045 359.309 5.8042 6.07985 0.302741 -0.025372988
14 0 0.961269005 -2.134582083 1.947021757 319.934 37.1911 0 0 300.332 154.252 4.17235 3.88519 61 61 446.033 226.508 448 223 31.5425 30.5006 268 268 56.7836 71.75 56 71 6.0179 8.76285 0 0 0 0 0 0 0 0 0 0 291.907 156.497 4.0553 3.94172 1 1 0 300.332 154.252 4.17235 3.88519 0.434707 0.961269005
15 0 0.424039288 0.276417169 1.341678761 339.99 39.5459 0 0 4659.06 3472.56 52.1458 53.1101 60 60 4802.03 3508.7 4884 3642 402.527 407.016 260 260 61.4038 74.5423 60 74 9.19724 10.1134 0 0 0 0 0 0 0 0 0 0 4643.29 3435.9 51.9692 52.5493 1 1 1 4659.06 3472.56 52.1458 53.1101 0.92911 0.424039288
16 0 -0.103960925 -0.154355823 0.930474859 361.946 38.9561 0 0 985.224 1058.84 10.9099 15.0311 60 60 1137.85 1117.55 1131 1121.5 84.2007 115.07 271 271 58.2435 72.0332 58 72 6.33799 9.51737 0 0 0 0 0 0 0 0 0 0 982.264 1047.26 10.8771 14.8667 1 1 1 985.224 1058.84 10.9099 15.0311 0.685143 -0.103960925
17 0 -0.001612954 -1.32015864 0.99888261 383.278 38.2776 0 0 281.592 281.907 6.5422 5.78903 62 62 434.306 361.177 434.5 355 49.8508 45.8921 281 281 63.2705 76.9075 62 75 9.9275 12.1575 0 0 0 0 0 0 0 0 0 0 273.694 286.008 6.3587 5.87325 1 1 1 281.592 281.907 6.5422 5.78903 0.425424 -0.001612954
21 0 0.884514157 -2.336382595 1.846142808 467.777 38.1603 0 0 321.635 174.22 4.65247 4.12172 62 62 468.823 245.823 464.5 244.5 35.4842 32.6625 269 269 58.9257 70.7732 59 71 5.95772 8.77181 0 0 0 0 0 0 0 0 0 0 312.554 176.788 4.52112 4.18247 1 1 0 321.635 174.22 4.65247 4.12172 0.475561 0.884514157
23 0 -0.190836818 0.966517558 0.876097404 510.28 38.8552 0 0 2936.88 3352.23 34.1978 44.0208 62 62 3104.61 3394.13 3063 3360 268.56 342.56 275 275 77.3927 82.3709 77 82 13.0123 12.9868 0 0 0 0 0 0 0 0 0 0 2929.88 3313.5 34.1162 43.5122 1 1 1 2936.88 3352.23 34.1978 44.0208 0.861139 -0.190836818
25 0 0.501848053 0.545240861 1.416026292 552.725 38.7025 0 0 1783.74 1259.68 15.5581 18.6636 60 60 1936.48 1316.18 1932 1300 120.222 142.786 274 274 59.1533 73.1423 59 72 6.37519 9.64867 0 0 0 0 0 0 0 0 0 0 1779.99 1244.78 15.5253 18.4428 1 1 1 1783.74 1259.68 15.5581 18.6636 0.52915 0.501848053
26 0 -1.269627311 0.530755306 0.414766905 574.412 37.5882 0 0 227.077 547.481 4.29054 8.84574 56 56 378 625.411 377.5 628.5 31.1366 66.8536 270 270 59.4259 73.0185 59 72 6.58883 9.70243 0 0 0 0 0 0 0 0 0 0 221.232 554.131 4.18009 8.95319 1 1 1 227.077 547.481 4.29054 8.84574 0.418128 -1.269627311
28 0 0.894410381 -2.988170177 1.858850031 616.681 37.7909 0 0 330.076 177.57 5.82951 4.18712 56 56 477.607 251.179 479.5 249 42.2726 31.5221 267 267 59.5393 72.7116 59 72 6.83268 9.13573 0 0 0 0 0 0 0 0 0 0 320.725 180.205 5.66437 4.24925 1 1 0 330.076 177.57 5.82951 4.18712 0.444134 0.894410381
29 0 0.142854351 -2.553680024 1.104087377 637.026 38.9017 0 0 305.183 276.412 5.12211 6.27047 56 56 452.446 349.857 449 337 37.1473 47.3872 255 255 58.4078 71.2314 58 70 6.2976 9.47756 0 0 0 0 0 0 0 0 0 0 296.696 280.364 4.97966 6.36013 1 1 1 305.183 276.412 5.12211 6.27047 0.513247 0.142854351

Total number of rows: 19061

Table truncated, full table size 5553 Kbytes.




Supplementary file Size Download File type/resource
GSM34074.tif.gz 23.9 Mb (ftp)(http) TIFF

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