|
| Status |
Public on Nov 08, 2018 |
| Title |
HSR066: Mst1/Mst2-KO_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
CD4+YFP+Treg cells from Foxp3YFP-CreStk4f/fStk3f/f mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: CD4+YFP+Treg cells
genotype: Mst1/Mst2-null
|
| Treatment protocol |
Sorted cells were lysed with lysis buffer from Rneasy Micro kit from Qiagen.
|
| Growth protocol |
CD45.2+CD45.1⎽CD4+YFP+ cells were sorted from bone marrow chimeras of RAG1-/- recipient mice transplanted 2 month prior with bone marrow from either Foxp3YFP-Cre or Foxp3YFP-CreStk4f/fStk3f/f mice togther with WT CD45.1 mice (CD45.2+:CD45.1+=1:1)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were prepared according to the prototol of Rneasy Micro kit from Qiagen,
|
| Label |
biotin
|
| Label protocol |
Total RNA (1 - 20 ng) were converted into biotinylated cDNA using the NuGEN WTA Pico v2 protocol and the NuGEN Encore Biotin module
|
| |
|
| Hybridization protocol |
Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix Mouse Gene 2.0 ST GeneChip. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console software v1.1
|
| |
|
| Submission date |
Oct 01, 2018 |
| Last update date |
Nov 08, 2018 |
| Contact name |
Geoffrey Neale |
| E-mail(s) |
geoffrey.neale@stjude.org
|
| Organization name |
St Jude Childrens Research Hospital
|
| Department |
Hartwell Center
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE120711 |
Hippo kinases Mst1 and Mst2 sense and amplify IL-2R−STAT5 signaling in Treg cells to establish stable regulatory activity |
|
