|
| Status |
Public on Oct 21, 2024 |
| Title |
Contr MSPCS 2 Bones_5: WT mouse mesenchymal stem and progenitor cells, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
WT mouse mesenchymal stem and progenitor cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mesenchymal stem and progenitor cells
tissue: Bones
genotype: WT
|
| Treatment protocol |
Rosa26Cre;LSL-KrasG12D mice and their single allele littermates were treated with a single dose of 1mg of tamoxifen via oral gavage to induce Cre-mediated KrasG12D expression. After 5.5 weeks, mice were sacrificed by cervical dislocation under anesthesia. Bone marrow niche cells were isolated out of bone and bone marrow cell suspensions using fluorescence-activating cell sorting.
|
| Growth protocol |
Rosa26Cre;LSL-KrasG12D mice and their single allele littermates were raised in the animal facility of the University Medical Center of Freiburg
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from bone marrow niche cells. RNA isolation was performed using the Qiagen AllPrep DNA/RNA Micro Kit according to the manufacturer’s protocol
|
| Label |
Biotin
|
| Label protocol |
We used the Affymetrix WT Pico kit for cDNA synthesis, amplification, and second-cycle cDNA synthesis, as well as fragmentation and labeling with Biotin, as described by the manufacturer.
|
| |
|
| Hybridization protocol |
Labeled fragments were hybridized to Affymetrix Mouse Gene ST 1.0 arrays for 16h at 45°C with 60 rpm in an Affymetrix Hybridization oven 645.
|
| Scan protocol |
After washing and staining, the arrays were scanned with the Affymetrix GeneChip Scanner 3000 7G. CEL-files were produced from the raw data with Affymetrix GeneChip Command Console Software.
|
| Description |
Gene expression from WT mouse mesenchymal stem and progenitor cells
|
| Data processing |
CEL files were analysed using the oligo R package. RMA method, log2 transformed were used to normalized the probe intensities.
|
| |
|
| Submission date |
Oct 07, 2018 |
| Last update date |
Oct 21, 2024 |
| Contact name |
Geoffroy Andrieux |
| Organization name |
University clinics Freiburg
|
| Street address |
Breisacherstr 153
|
| City |
Freiburg |
| ZIP/Postal code |
79110 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE120912 |
Oncogenic Kras G12D activation in the non-hematopoietic bone marrow microenvironment causes myelodyplastic syndrom in mice |
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