|
| Status |
Public on May 28, 2009 |
| Title |
ES_1283_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
elongating stem from genotype 1283
|
| Organism |
Medicago sativa |
| Characteristics |
Genotype 1283.
The internodes immediately above the transition internode.
|
| Treatment protocol |
No treatment.
|
| Growth protocol |
The alfalfa clonal lines 252 and 1283 were propagated from cuttings and grown in the greenhouse. The greenhouse experiments consisted of three replicates arranged in a randomized complete block design. There were eight plants of each clone, in individual pots, in each replicate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Medicago Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from elongating stem of alfalfa genotype 1283.
|
| Data processing |
Cel files were analyzed by GCOS1.4 with scale factor of 1.0, without normalization.
|
| |
|
| Submission date |
Nov 13, 2008 |
| Last update date |
May 28, 2009 |
| Contact name |
John W Gronwald |
| E-mail(s) |
John.Gronwald@ars.usda.gov
|
| Phone |
6126258186
|
| Fax |
6516495058
|
| Organization name |
USDA-ARS
|
| Lab |
Gronwald
|
| Street address |
1991 UPPER BUFORD CIRCLE
|
| City |
SAINT PAUL |
| State/province |
MN |
| ZIP/Postal code |
55108-6026 |
| Country |
USA |
| |
|
| Platform ID |
GPL4652 |
| Series (1) |
| GSE13602 |
Transcriptome Profiles of Medicago Stem Internodes |
|
