|
| Status |
Public on Jun 02, 2009 |
| Title |
working myocardium _ stage e17.5 _ biorep5 |
| Sample type |
RNA |
| |
|
| Source name |
working myocardium, stage e17.5, biorep5
|
| Organism |
Mus musculus |
| Characteristics |
working myocardium
stage: E17.5
|
| Growth protocol |
normal development
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For every sample, transgenic hearts were dissected, the GFP-positive tissues pooled, dissociated to obtain single cells, and purified using FACS. 5000 cells per sample were gated and used for RNA isolation (Macherey-Nagel, nucleospin II). RNA was subjected to 2 rounds of linear amplification and biotin labeling (Ambion). Within each group 6 labeled antisense RNA samples were hybridized to separate Illumina Mouse-Ref-6 v1.1 BeadChips.
|
| Label |
biotin
|
| Label protocol |
RNA was subjected to 2 rounds of linear amplification and biotin labeling (Ambion). Within each group 6 labeled antisense RNA samples were hybridized to separate Illumina Mouse-Ref-6 v1.1 BeadChips.
|
| |
|
| Hybridization protocol |
standard Illumina hybridization protocol
|
| Scan protocol |
standard Illumina hybridization protocol
|
| Description |
W5_E17.5
|
| Data processing |
Data were processed using the R/Limma package (Bioconductor). Data were normalized using VSN transformation.
|
| |
|
| Submission date |
Nov 14, 2008 |
| Last update date |
May 28, 2009 |
| Contact name |
Henk Buermans |
| E-mail(s) |
h.buermans@lumc.nl
|
| Phone |
+31715268558
|
| Organization name |
Leiden University Medical Center
|
| Department |
Human Genetics
|
| Lab |
Leiden Genome Technology Center
|
| Street address |
Albinusdreef 2
|
| City |
Leiden |
| State/province |
ZH |
| ZIP/Postal code |
2300 RC |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE13614 |
Expression profiling of the forming atrioventricular node using a novel Tbx3-based node-specific transgenic reporter |
|
