|
| Status |
Public on Jan 15, 2009 |
| Title |
delta-pbf1 delta-pbf2 cells at T0, replicate #3 |
| Sample type |
RNA |
| |
|
| Source name |
S. cerevisiae cells before heat shock
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
delta-pbf1 delta-pbf2
|
| Treatment protocol |
Half of the cells were collected at T0 and half of the cells were subjected to 25 ºC-->37 ºC heat shock and cells were collected after 20 min, as samples T20.
|
| Growth protocol |
S. cerevisiae cells were grown @ 25 ºC to O.D. 600 of 0.3~04, in triplicate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RiboPure kit were used to extract total RNA. Total RNAs were further purified using Qiagen RNAeasy Mini kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 300.
|
| Description |
n/a
|
| Data processing |
MAS 5
|
| |
|
| Submission date |
Nov 20, 2008 |
| Last update date |
Jan 15, 2009 |
| Contact name |
Cong Zhu |
| E-mail(s) |
cong.zhu@umassmed.edu
|
| Phone |
508-856-3756
|
| Organization name |
UMass Medical School
|
| Department |
Program in Gene Function and Expression
|
| Street address |
364 Plantation Street
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE13684 |
High-Resolution DNA Binding Specificity Analysis of Yeast Transcription Factors |
|
