|
| Status |
Public on Apr 09, 2019 |
| Title |
KPB1-B CC 189 Untreated |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
common reference RNA (consisted of total RNA harvested from equal numbers of C57Bl6/J and 129 male and female day 1 pups. Reference to original publication PMID:17493263)
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6/J and 129
sample type: Reference
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated using Qiagen RNeasy Kit
|
| Label |
Cy3
|
| Label protocol |
described in Hu et al., 2005, BioTechniques, 38:121-125
|
| |
|
| Channel 2 |
| Source name |
KPB1-B CC 189 Untreated
|
| Organism |
Mus musculus |
| Characteristics |
treatment: None
time: 0 days
line: tumor transplant line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated using Qiagen RNeasy Kit
|
| Label |
Cy5
|
| Label protocol |
described in Hu et al., 2005, BioTechniques, 38:121-125
|
| |
|
| |
| Hybridization protocol |
described in Hu et al., 2005, BioTechniques, 38:121-125
|
| Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with an Agilent scanner and image intensity data were extracted and analyzed with Agilent Feature Extraction software
|
| Description |
Transplant
|
| Data processing |
Data for both channels were Lowess-normalized and then the log(2) ratio was taken
|
| |
|
| Submission date |
Nov 01, 2018 |
| Last update date |
Apr 09, 2019 |
| Contact name |
Charles M. Perou |
| E-mail(s) |
cperou@med.unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Professor of Genetics, and Pathology & Laboratory Medicine; Lineberger Comprehensive Cancer Center
|
| Street address |
12-044 Lineberger Comprehensive Cancer Center CB# 7295
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7264 |
| Country |
USA |
| |
|
| Platform ID |
GPL24315 |
| Series (2) |
| GSE122075 |
A mouse model featuring tissue specific deletion of p53 and Brca1 gives rise to mammary tumors with genomic and transcriptomic similarities to human basal-like breast cancer [expression] |
| GSE122076 |
A mouse model featuring tissue specific deletion of p53 and Brca1 gives rise to mammary tumors with genomic and transcriptomic similarities to human basal-like breast cancer |
|
