|
| Status |
Public on Jan 01, 2020 |
| Title |
Na2SeO3+CYP rep1 |
| Sample type |
SRA |
| |
|
| Source name |
liver tissue
|
| Organism |
Mus musculus |
| Characteristics |
treatment: 40mg/kg [beta]-CYP and 50μg/kg Na2SeO3
tissue: liver
strain: Swiss
ages: weeks 10-12
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent.TruSeq® RNA LT Sample Prep Kit v2 was used with total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard BGISEQ500 protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
gene_exp_with_symbol.txt
processed data column header: f1
|
| Data processing |
BGISEQ500 platform used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm9 whole genome using HISAT software
Exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by Cufflinks
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include the expression values of this transcript for each Sample ..
|
| |
|
| Submission date |
Nov 05, 2018 |
| Last update date |
Jan 01, 2020 |
| Contact name |
Kan He |
| E-mail(s) |
hekan@ahu.edu.cn
|
| Organization name |
Anhui University
|
| Department |
School of life sciences
|
| Lab |
Center for Stem Cell and Translational Medicine
|
| Street address |
111 Jiulong Road
|
| City |
Hefei |
| State/province |
Anhui |
| ZIP/Postal code |
230601 |
| Country |
China |
| |
|
| Platform ID |
GPL21493 |
| Series (1) |
| GSE122165 |
A transcriptomic study of selenium against liver injury by RNA-seq |
|
| Relations |
| BioSample |
SAMN10380327 |
| SRA |
SRX4980109 |
