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Status |
Public on Nov 05, 2023 |
Title |
E9_2 |
Sample type |
RNA |
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|
Source name |
Healthy Donor 2
|
Organism |
Homo sapiens |
Characteristics |
individual: donor 2 disease state: healthy tissue: Peripheral blood from healthy donor cell type: CD36- SLAN+ monocytes
|
Treatment protocol |
PBMCs were isolated by Ficoll-Paque gradient as described elsewhere. Purified PBMCs were stained with the 7-color mAb combination - HLADR-PacB, CD45-OC515, CD36-FITC, SLAN-PE, CD16-PECy7, CD300e-APC, CD14APCH7 - with FSCintSSCintCD14lo/-CD36+/-CD45++CD300e+HLADR+ intermediate and non-classical monocytes being FACSorted into SLAN- and SLAN+ cells.
|
Growth protocol |
Cells were directly purified from fresh peripheral blood from healthy donors
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Extracted molecule |
total RNA |
Extraction protocol |
The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
|
Label |
Biotin
|
Label protocol |
The sense cDNA was prepared from 300 ng of total RNA using the Ambion® WT Expression Kit. The sense strand cDNA was then fragmented and biotinylated with the Affymetrix GeneChip® WT Terminal Labeling Kit (PN 900671).
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Hybridization protocol |
Labeled sense cDNA was hybridized to the Affymetrix HuGene-2_0-st chip according to the the manufacturer protocols and using GeneChip® Hybridization, Wash and Stain Kit.
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Scan protocol |
Genechips were scanned with the Affymetrix GeneChip® Scanner 3000.
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Description |
Gene expression data from CD36- SLAN+ monocytes
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Data processing |
Data was normalized using Bioconductor software and RMA algorithm.
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|
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Submission date |
Nov 05, 2018 |
Last update date |
Nov 05, 2023 |
Contact name |
Aintzane Zabaleta |
E-mail(s) |
azabaletaa@unav.es
|
Organization name |
FOUNDATION FOR APPLIED MEDICAL RESEARCH
|
Street address |
Avda. Pío XII 55
|
City |
Pamplona |
State/province |
Navarra |
ZIP/Postal code |
31008 |
Country |
Spain |
|
|
Platform ID |
GPL16686 |
Series (1) |
GSE122171 |
Transcriptomic analysis of SLAN+ vs SLAN- non-classical monocytes |
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