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Status |
Public on Aug 01, 2009 |
Title |
H3Cter binding wild type cells KCl 60 min |
Sample type |
genomic |
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|
Source name |
S. pombe strain Fy368 grown to mid log phase in YES at 25°C, then treated with KCl (final concentration 1M) 60 min
|
Organism |
Schizosaccharomyces pombe |
Characteristics |
genotype: h-, leu1-32, ura4-D18, ade6-M210
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Treatment protocol |
Treatment for each sample is described in "source name"
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Growth protocol |
Yeast cells grow at 25°C in YES medium to log phase.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Experimental procedure was essentially according to Robyr and Grunstein, Method 31 (2003) 83-89
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Label |
biotin
|
Label protocol |
Biotinylated genomic DNA were prepared according to the standard Affymetrix protocol: affymetrix chromatin immunoprecipitation assay protocol. 2005-2006
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|
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Hybridization protocol |
Fragmented genomic DNA was hybridized to Affymetrix GeneChip S. pombe Tiling 1.0FR according to Affymetrix chromatin immunoprecipitation assay protocol. 2005-2006
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Scan protocol |
According to affymetrix chromatin immunoprecipitation assay protocol. 2005-2006
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Description |
immunoprecipitated material using antibodies against Histone H3 (H3Cter)
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Data processing |
Tiling analysis software (TAS) from Affymetrix
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|
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Submission date |
Dec 02, 2008 |
Last update date |
Jan 22, 2009 |
Contact name |
Anthony Wright |
E-mail(s) |
yongtao.xue.franzen@ki.se
|
Organization name |
Karolinska Institute
|
Lab |
Anthony Wright
|
Street address |
Blickagången 6
|
City |
Huddinge |
ZIP/Postal code |
141 57 |
Country |
Sweden |
|
|
Platform ID |
GPL7715 |
Series (1) |
GSE13790 |
Specific acetylase-deacetylase balance controls stress adaptation by modulating global histone H3K14 acetylation. |
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