|
| Status |
Public on Aug 01, 2009 |
| Title |
H3Cter binding wild type cells KCl 60 min |
| Sample type |
genomic |
| |
|
| Source name |
S. pombe strain Fy368 grown to mid log phase in YES at 25°C, then treated with KCl (final concentration 1M) 60 min
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
genotype: h-, leu1-32, ura4-D18, ade6-M210
|
| Treatment protocol |
Treatment for each sample is described in "source name"
|
| Growth protocol |
Yeast cells grow at 25°C in YES medium to log phase.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Experimental procedure was essentially according to Robyr and Grunstein, Method 31 (2003) 83-89
|
| Label |
biotin
|
| Label protocol |
Biotinylated genomic DNA were prepared according to the standard Affymetrix protocol: affymetrix chromatin immunoprecipitation assay protocol. 2005-2006
|
| |
|
| Hybridization protocol |
Fragmented genomic DNA was hybridized to Affymetrix GeneChip S. pombe Tiling 1.0FR according to Affymetrix chromatin immunoprecipitation assay protocol. 2005-2006
|
| Scan protocol |
According to affymetrix chromatin immunoprecipitation assay protocol. 2005-2006
|
| Description |
immunoprecipitated material using antibodies against Histone H3 (H3Cter)
|
| Data processing |
Tiling analysis software (TAS) from Affymetrix
|
| |
|
| Submission date |
Dec 02, 2008 |
| Last update date |
Jan 22, 2009 |
| Contact name |
Anthony Wright |
| E-mail(s) |
yongtao.xue.franzen@ki.se
|
| Organization name |
Karolinska Institute
|
| Lab |
Anthony Wright
|
| Street address |
Blickagången 6
|
| City |
Huddinge |
| ZIP/Postal code |
141 57 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL7715 |
| Series (1) |
| GSE13790 |
Specific acetylase-deacetylase balance controls stress adaptation by modulating global histone H3K14 acetylation. |
|
