|
| Status |
Public on Dec 04, 2018 |
| Title |
bion_flg22_rep1_array9.3 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis thaliana L. Heynh cultivar 6 Columbia (Col-0)
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissus: Four whole plants (leaves)
genetic background: Arabidopsis thaliana L. Heynh cultivar 6 Columbia (Col-0)
genotype: wild type
phenotype: wild type
treatment: bion_flg22
age: 21 days after germination
growth protocol: long day
harvest date: january 2016
|
| Treatment protocol |
Four Arabidopsis plants represent one biological replicate. Plants were treated with BTH by spraying with final concentrations of 1mM. Plants were treated the first time 7 days after germination and the second time 10 days after germination. RNA was extracted 21 days after germination.
|
| Growth protocol |
Plant material used was wild-type Arabidopsis thaliana L. Heynh cultivar 6 Columbia (Col-0). All Arabidopsis plants were grown under long day condition (photoperiod of 16h light at 22°C/ 8h light at 22°C, with 60% relative humidity).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was extracted using the NucleoSpin RNA plant extraction kit (Machery-Nagel Hoerdt, France) according the manufacturer`s recommendations.
|
| Label |
Cy3
|
| Label protocol |
Amplification and labbeling were performed with MessageAmp™ II aRNA Amplification Kit (Ambion) with Cy5/Cy3 labels
|
| |
|
| Hybridization protocol |
The hybridization performed on a rotative Agilent Hybridization Oven, 17h at 65°C, 10g. Afterwards, the slide were washed, and scanned.
|
| Scan protocol |
Scanning was performed on 2µm NimbleGen MS 200 scanner
|
| Description |
Raw Data: Cy3: 257111410009_Arabido009_scan_2016-01-19_532_635_GE2_1105_Oct12_1_3.txt
|
| Data processing |
All statistical analyses on the gene expression data were performed using the R language, version 2.5.1 (R Development Core Team, 2011) and the package LIMMA (Smyth G.K., 2005) from the Bioconductor project. For the processing step, data were normalized by the lowess method in LIMMA.
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| |
|
| Submission date |
Dec 03, 2018 |
| Last update date |
Dec 04, 2018 |
| Contact name |
Sandra Pelletier |
| E-mail(s) |
sandra.pelletier@inra.fr
|
| Organization name |
INRA
|
| Lab |
IRHS
|
| Street address |
42, rue Georges Morel - BP 60057
|
| City |
BEAUCOUZE |
| ZIP/Postal code |
49045 |
| Country |
France |
| |
|
| Platform ID |
GPL25797 |
| Series (1) |
| GSE123073 |
Biotic stress-induced priming and de-priming of transcriptional memory in Arabidopsis |
|
