|
| Status |
Public on Dec 12, 2008 |
| Title |
Medicago truncatula seedlings, 2 wks hydroponics media, 200mM NaCl salt stress, 0 hour |
| Sample type |
RNA |
| |
|
| Source name |
Medicago truncatula seedlings, grown 2 wks in hydroponics media, 200mM NaCl salt stress, time point 0 hour
|
| Organism |
Medicago truncatula |
| Characteristics |
Medicago truncatula seedlings
grown two weeks in hydroponics media
200mM NaCl salt stress
2 ug of total RNA was used to synthesize doublestranded cDNA
|
| Biomaterial provider |
INRA Medicago truncatula Stock Center
|
| Treatment protocol |
200mM NaCl salt stress
|
| Growth protocol |
grown two weeks in hydroponics media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
using TRIzol reagent performed according to the manufacturer’s instruction
|
| Label |
biotin
|
| Label protocol |
using the Custom MessageAmp™ II-Biotin by Ambion according to the manufacturer’s instruction
|
| |
|
| Hybridization protocol |
performed at 45℃ with rotation for 16h (Affymetrix GeneChip Hybridization Oven 640)
|
| Scan protocol |
using Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data of roots of Medicago truncatula seedlings grown two weeks in hydroponics media under salt stress
|
| Data processing |
The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). The scanned images were first assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of GCOS 1.4.
|
| |
|
| Submission date |
Dec 11, 2008 |
| Last update date |
Dec 17, 2008 |
| Contact name |
Daofeng Li |
| E-mail(s) |
lidaof@gmail.com
|
| Phone |
(314) 286-0866
|
| Organization name |
Washington University in St. Louis
|
| Department |
Genetics
|
| Lab |
Ting Wang Lab
|
| Street address |
4515 McKinley Avenue
|
| City |
SAINT LOUIS |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL4652 |
| Series (2) |
| GSE13907 |
Medicago truncatula seedlings grown two weeks in hydroponics media then carry out salt stress |
| GSE14029 |
Effects of salt stress on Medicago truncatula seedlings |
|
