|
| Status |
Public on Jan 10, 2009 |
| Title |
Chlorhexidine diacetate_60min_replicate3 |
| Sample type |
RNA |
| |
|
| Source name |
Pseudomonas aeruginosa_Chlorhexidine diacetate
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
PA01 Wild type
|
| Biomaterial provider |
American Type Culture Collection (ATCC)
|
| Treatment protocol |
Chlorhexidine diacetate (0.008mM)
|
| Growth protocol |
(i) we initiated P. auruginosa cultures at 37°C with shaking at 250 rpm using sterilized Luria-Bertani (LB) broth, (ii) after 17 hours, we diluted the overnight cultures 1:100 in pre-warmed LB broth and incubated at 37°C with shaking at 250 rpm until OD600 reached the early logarithmic phase (~ 0.8), and (iii) we re-diluted the cells 1:10 in pre-warmed LB broth and incubated at 37°C with shaking at 250 rpm. Then, we added 0.008 mM chlorhexidine diacetate immediately after OD600 reached 0.8.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated at 60 min after OD600 reached 0.8 using the Rneasy Mini Kit (Qiagen Inc., Valencia, CA) according to the manufacturer’s protocol.
|
| Label |
Biotin
|
| Label protocol |
Approximately 12 µg of total RNA was processed to produce biotinylated cDNA targets.
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures (target signal value was 500)
|
| Description |
Chlorhexidine diacetate treatment
|
| Data processing |
Affymetrix GeneChip Opererating Software (GCOS) version 1.2.
|
| |
|
| Submission date |
Dec 31, 2008 |
| Last update date |
Jan 06, 2009 |
| Contact name |
Chantal Wheko Nde |
| Organization name |
USEPA
|
| Department |
OPP/BEAD
|
| Lab |
MARL
|
| Street address |
701 Mapes Road
|
| City |
Fort Meade |
| State/province |
MD |
| ZIP/Postal code |
20755 |
| Country |
USA |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE14253 |
Microarray Analysis of Toxicogenomic Effects of Chlorhexidine diacetate on Pseudomonas aeruginosa |
|
