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| Status |
Public on Apr 15, 2019 |
| Title |
Zikv-Aag2 (small RNA) |
| Sample type |
SRA |
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| Source name |
Aedes aegypti Aag-2 cells
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| Organism |
Aedes aegypti |
| Characteristics |
cell type: Aag2
infection: Zika virus
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| Extracted molecule |
total RNA |
| Extraction protocol |
TruSeq Small RNA Library Preparation Kits (Illumina)
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| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
The sequencing reads were mapped to the human genome (release hg19) or mosquito genome (AaeGL5.0) by using Bowite 2.2.5 and the cellular miRNA expression level was determined on the
basis of the number of reads that was identical to the annotated miRNA sequences in miRBase version 18, only allowing −2 or +2 nt to be templated by the corresponding genomic sequence at the 3′ end. Viral small RNA (vsRNA) reads which cannot be mapped to human genome were then mapped to ZIKV genome with 100% identity. The subsequent analysis of length and location of these vsRNAs were calculated using in-house Perl scripts. Pairs of complementary
22-nt vsRNAs in each library in different distance categories were computed using principles as previously described(Li et al., 2016; Li et al., 2013).
Genome_build: hg19, AaeGL5.0
Supplementary_files_format_and_content: txt files generated by bowtie2
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| Submission date |
Jan 23, 2019 |
| Last update date |
Apr 16, 2019 |
| Contact name |
xi zhou |
| E-mail(s) |
zhouxi@wh.iov.cn
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| Organization name |
Wuhan University
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| Department |
State Key Laboratory of Virology, College of Life Sciences
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| Street address |
Luojia Hill
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430072 |
| Country |
China |
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| Platform ID |
GPL21020 |
| Series (1) |
| GSE125520 |
Zika virus infection induces RNAi-mediated antiviral immunity in human neural progenitors and brain organoids |
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| Relations |
| BioSample |
SAMN10793660 |
| SRA |
SRX5283411 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM3576325_Zika-Aag2_21nt_vsRNAs.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| GSM3576325_Zika-Aag2_22nt_vsRNAs.txt.gz |
87.0 Kb |
(ftp)(http) |
TXT |
| GSM3576325_Zika-Aag2_23nt_vsRNAs.txt.gz |
25.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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