|
| Status |
Public on Feb 28, 2009 |
| Title |
C.elegans_Temperature 25 degrees_1 |
| Sample type |
RNA |
| |
|
| Source name |
25 degrees
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
Ceanorhabditis elegans, N2 wild type
|
| Growth protocol |
The Bristol N2 strain was pre-cultured in C. elegans maintenance medium (CeMM) for 1 month at 20 °C. Approximately 5000 worms of mixed larval stage were transferred into 5 mL culture bags containing fresh CeMM at 10 for 2 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Worms were homogenized with four rounds of 20-second beating at 5000 rpm in a Micro SmashTM MS-100 bead beater with 3 minutes of cooling on ice between rounds. Total RNA was extracted using an Isogen RNA purification kit following the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
Total RNA was amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit protocol. Cy3 labeled cRNA was produced according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Labeled cRNA (1.65 µg/sample) was hybridized to a C. elegans oligo microarray using a gene expression hybridization kit at 60 °C for 17 hours.
|
| Scan protocol |
Chips were scanned with an Agilent Scanner and processed with Agilent Feature Extraction Software (Version 9.1.3.1).
|
| Description |
This study focused on temperature, profiling the altered gene expression that develops under four thermal conditions (10, 15, 20, and 25°C) in C. elegans.
|
| Data processing |
Microarray data were analyzed using Gene Spring software (Agilent Technologies). Log ratios are derived by per Chip (Normalize to 50th percentile) and per Gene.
|
| |
|
| Submission date |
Jan 14, 2009 |
| Last update date |
Jan 23, 2009 |
| Contact name |
Akira Higashibata |
| E-mail(s) |
higashibata.akira@jaxa.jp
|
| Phone |
050-3362-3898
|
| Organization name |
Japan Aerospace Exploration Agency
|
| Department |
Department of Space Biology and Microgravity Sciences
|
| Lab |
ISS Science Project office
|
| Street address |
2-1-1,Sengen
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8505 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7727 |
| Series (1) |
| GSE14475 |
Gene expression profiling of C. elegans under various thermal conditions |
|
