|
| Status |
Public on Aug 01, 2019 |
| Title |
RAP, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Rapid atrial pacing, pair #3
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: left atrium
gender: Male
strain: Sprague-Dawley
age: adult
treatment: exposed to rapid atrial pacing for 48 hours
|
| Treatment protocol |
Rats were subjected to either rapid atrial pacing (RAP, 70 ms Cycle length) or control pacing (Con, 140 ms Cycle length) for 48 hours. Ventricular rate was simillar in both groups
|
| Growth protocol |
Male Sprague-Dawley rats (250-320 gr)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from the left atrium with TRI-Reagent (MRC, Cincinnati, OH, USA)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA from each sample
|
| |
|
| Hybridization protocol |
The biotinylated Samples were hybridized to the GeneChip Rat Gene 1.0 ST arrays contains 722,254 probe sets (for estimated 27,342 genes).
|
| Scan protocol |
Following hybridization the arrays were washed and stained using FS450_0007 protocol, and fluorescent signals were scanned using the 3000 G7 Scanner (Affymetrix)
|
| Description |
Gene expression data from left atria exposed to rapid atrial pacing for 48 hours
|
| Data processing |
CEL files were processed in Partek Genomics Suite® using Robust Multiarray Averaging (RMA, Irizarry et al 2003) with default parameters.
|
| |
|
| Submission date |
Feb 18, 2019 |
| Last update date |
Aug 01, 2019 |
| Contact name |
Yoram Etzion |
| E-mail(s) |
tzion@bgu.ac.il
|
| Phone |
972-86479987
|
| Organization name |
Ben-Gurion University, Israel
|
| Department |
Physiology and Cell Biology
|
| Lab |
Cardiac Arrhythmia Research Lab
|
| Street address |
1st Ben-Gurion Street
|
| City |
Beer-Sheva |
| State/province |
Please Select |
| ZIP/Postal code |
8410501 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE126711 |
Left atrial tissue of rats exposed to rapid atrial pacing |
|
