|
| Status |
Public on Feb 26, 2019 |
| Title |
PolyIC-stimulated MPs - 6 days + Gardiquimod 24 h [DC_pIC-G-2] |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
PolyIC-stimulated MPs - 6 days + Gardiquimod 24 h
|
| Organism |
Salmo salar |
| Characteristics |
treatment: PolyIC-stimulated MPs - 6 days + Gardiquimod 24 h
|
| Growth protocol |
Primary salmon mononuclear phagocytes (MPs) isolated from head kidney and cultured in vitro in L-15 medium supplemented with 5% FBS for up to 7 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy® Mini Kit (Qiagen)
|
| Label |
Cy5
|
| Label protocol |
Amplified and labeled with Low Input Quick Amp Labeling Kit (Agilent).
|
| |
|
| Channel 2 |
| Source name |
control macriphages - 1 day
|
| Organism |
Salmo salar |
| Characteristics |
treatment: Control
|
| Growth protocol |
Primary salmon mononuclear phagocytes (MPs) isolated from head kidney and cultured in vitro in L-15 medium supplemented with 5% FBS for up to 7 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy® Mini Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Amplified and labeled with Low Input Quick Amp Labeling Kit (Agilent).
|
| |
|
| |
| Hybridization protocol |
Hybridized according to Agilent Two-Color Microarray-Based Gene Expression Analysis protocol for 4x44k microarrays
|
| Scan protocol |
Arrays were scanned with GenePix 4100A microarray scanner
|
| Data processing |
Assessment of spot quality was done with aid of GenePix flags. After filtration of low quality spots, Lowess normalization of log2-expression ratios was performed.
|
| |
|
| Submission date |
Feb 25, 2019 |
| Last update date |
Feb 26, 2019 |
| Contact name |
Aleksei Krasnov |
| E-mail(s) |
aleksei.krasnov@nofima.no
|
| Phone |
+47 97602165
|
| Organization name |
Nofima AS
|
| Department |
Fish Health
|
| Street address |
Osloveien 1
|
| City |
Aas |
| ZIP/Postal code |
1430 |
| Country |
Norway |
| |
|
| Platform ID |
GPL15678 |
| Series (1) |
| GSE126993 |
CpGs induce differentiation of Atlantic salmon mononuclear phagocytes into cells with dendritic morphology and a proinflammatory transcriptional profile but an exhausted allostimulatory activity |
|
