|
| Status |
Public on Jan 31, 2009 |
| Title |
CD4 T sells, Self Antigen, Rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Self-antigen model (C3HA)
|
| Organism |
Mus musculus |
| Characteristics |
B10.d2, Male,CFSE-diluted , Self-antigen activated CD4 T cell
|
| Treatment protocol |
HA-specific CD4 T that had undergone antigen specific division in vivo (i.e. CFSE-diluted)cells were harvested from lymph nodes 3-7 days post adoptive transfer. FACSVantage SE cell sorter (BD Biosciences) was used to sort T cells by gating on CFSE-diluted and Thy1.1+ (donor origin) from enriched CD4 T cells
|
| Growth protocol |
CFSE labeled TCR transgenic CD4 T cells specific for HA were harvested from donor mice, purified using Miltenyi’s magnetically labeled beads, and adoptively transferred into tumor-antigen recognition model (ProHA x TRAMP), Self-antigen recognition model (C3HA), viral-antigen recognition model (VaccHA) activation control, and naïve adoptive transfer control (Nontrangenic) mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sorted cells were pelleted suspended in Trizol and stored at -80ºC prior to RNA extraction using the Trizol reagent according to the manufacturer's instructions. RNA integrity was analyzed using an Agilent 2100 Bioanalyzer, the RNA 6000 Pico, and Nano Kits (Agilent Technologies).
|
| Label |
Biotin
|
| Label protocol |
Standard labeling following the manufacturers protocol was carried out after cDNA was synthesized using the Nugen FL-Ovation cDNA Biotin Module V2 kit, (http://www.nugeninc.com/pdfs/flbv2_userguide.pdf).
|
| |
|
| Hybridization protocol |
Samples were hybridized to separate Affymetrix Mouse 430 Plus2 expression array chips
|
| Scan protocol |
Expression array Chips were scanned using Affymetrix GeneChip Scanner 3000
|
| Description |
Gene Expression Profile of a T cell responding to self
CDra-TTT-HA-1a-MOE430_2 [C3HA]
|
| Data processing |
Relative expression levels were calculated using the MAS5.0 normalization
|
| |
|
| Submission date |
Jan 30, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Charles G Drake |
| E-mail(s) |
drakech@jhmi.edu
|
| Phone |
(410) 502-7523
|
| Fax |
(443) 287-4653
|
| Organization name |
Johns Hopkins Sidney Kimmel Comprehensive Cancer Center
|
| Department |
Department of Oncology
|
| Lab |
Immunology
|
| Street address |
1650 Orleans St CRB I #410
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21231 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE14662 |
Tumor and Self-Recognition Induce Distinct Transcriptional Profiles in Antigen-Specific CD4 T Cells |
|
| Relations |
| Reanalyzed by |
GSE119085 |
