|
| Status |
Public on Sep 15, 2020 |
| Title |
SVEC_HGF6h_3 |
| Sample type |
RNA |
| |
|
| Source name |
SVEC4-10 6h 20 ng/ml HGF
|
| Organism |
Mus musculus |
| Characteristics |
cell line: SVEC4-10
cell type: endothelial cells
timepoint: 6h
|
| Treatment protocol |
For HGF stimulation, SVEC4-10 were serum starved for 3h prior to supplementation of fresh starvation medium with/without HGF supplementation.
|
| Growth protocol |
SVEC4-10 were purchased from ATCC and cultivated according to the manufacturer's recommendations.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using NucleoSpin RNA II kit (Macherey Nagel) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
SVEC4-10 were serum staved for 3h and then further cultivated in fresh starvation medium with/without HGF supplementation
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 7, from SAS. Gene expression profiling was performed using arrays of mouse Mogene-2_0-type from Affymetrix. A Custom CDF Version 21 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Mar 08, 2019 |
| Last update date |
Sep 16, 2020 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23092 |
| Series (2) |
| GSE128046 |
YAP orchestrates heterotypic endothelial cell communication via HGF/c-MET signaling in liver tumorigenesis |
| GSE128047 |
An endothelial subtype-specific communication network orchestrates capillarization in liver tumorigenesis via the Hgf/c-Met pathway |
|
