|
| Status |
Public on Mar 01, 2009 |
| Title |
B.theta-E.rectale-High polysaccharide diet_#5 |
| Sample type |
RNA |
| |
|
| Source name |
B.theta and E.rectale growing in the cecum of mice fed a high polysaccharide diet
|
| Organisms |
Bacteroides thetaiotaomicron; Agathobacter rectalis |
| Characteristics |
strain: NMRI
gender: male
age: 14 weeks
diet: high polysaccharide
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy column and Ambion DNAfree DNAseI treatment
|
| Label |
Biotin
|
| Label protocol |
Enzo Biochemicals Bioarray terminal labeling kit
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
B. thetaiotaomicron and Eubacterium rectale, grown in vivo in the cecum of NMRI inbred mouse fed a plant polysaccharide diet.
|
| Data processing |
Expression analyses were performed using the MAS5 algorithm implemented in BioConductor’s ‘Affy package’, following masking of GeneChip imperfections with Harshlight - in both cases using the default parameters.
|
| |
|
| Submission date |
Feb 03, 2009 |
| Last update date |
Feb 05, 2009 |
| Contact name |
Federico E Rey |
| E-mail(s) |
reyf@wustl.edu
|
| Phone |
314-963-0284
|
| Organization name |
Washington University
|
| Department |
Pathology
|
| Lab |
Gordon
|
| Street address |
4444 Forest Park Blvd
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
|
| Platform ID |
GPL7006 |
| Series (1) |
| GSE14709 |
Transcriptional profile of B. theta and E. rectale growing in the cecum of mice fed different diets |
|
