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Sample GSM372721 Query DataSets for GSM372721
Status Public on May 20, 2009
Title Healthy_CD4_1
Sample type RNA
 
Source name CD4 T cells
Organism Homo sapiens
Characteristics gender: male
age: 30
t_cell_purity: 89%
fab_type: Not applicable
prognostic_group: Not applicable
Extracted molecule total RNA
Extraction protocol T cells were isolated by immunomagnetic selection. RNA was then extracted using Trizol.
Label biotin
Label protocol Affymetrix Expression GeneChip Protocol one-cycle procedure. Between 2 and 5micrograms of RNA was used as starting material.
 
Hybridization protocol Following fragmentation, 15 micrograms of cRNA were hybridised for 16 hours at 45C on Affymetrix U133Plus2 GeneChips. Chips were then washed and stained with SAPE in an Affymetrix GeneChip 450 Fluidics Station.
Scan protocol GeneChips were scanned using the Ayymetrix GeneChip 3000 scanner
Description Healthy1 CD4
Data processing Data were analysed with MicroArray Suite 5.0 (MAS 5.0) using Affymetrix default analysis settings.
 
Submission date Feb 20, 2009
Last update date Sep 01, 2016
Contact name Rifca Le Dieu
E-mail(s) rledieu@gmail.com
Organization name Queen Mary University of London
Department Institute of Cancer
Lab Centre for Medical Oncology
Street address 3rd Floor, John Vane Science Building, Charterhouse Square
City London
ZIP/Postal code EC1M 6BQ
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE14924 Characterisation of gene expression changes in T cells from patients presenting with AML compared with healthy T cells
Relations
Reanalyzed by GSE86362

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 268.908 P 0.000389797
AFFX-BioB-M_at 322.988 P 4.42873e-05
AFFX-BioB-3_at 178.636 P 4.42873e-05
AFFX-BioC-5_at 742.415 P 4.42873e-05
AFFX-BioC-3_at 918.931 P 4.42873e-05
AFFX-BioDn-5_at 1595.34 P 4.42873e-05
AFFX-BioDn-3_at 2885.26 P 5.16732e-05
AFFX-CreX-5_at 8830.28 P 5.16732e-05
AFFX-CreX-3_at 9830.23 P 4.42873e-05
AFFX-DapX-5_at 1.19202 A 0.631562
AFFX-DapX-M_at 13.4421 A 0.147939
AFFX-DapX-3_at 2.3345 A 0.891041
AFFX-LysX-5_at 9.91363 A 0.300606
AFFX-LysX-M_at 3.31449 A 0.631573
AFFX-LysX-3_at 1.10945 A 0.51489
AFFX-PheX-5_at 2.51485 A 0.760937
AFFX-PheX-M_at 1.97129 A 0.772364
AFFX-PheX-3_at 4.34376 A 0.58862
AFFX-ThrX-5_at 6.79861 A 0.574038
AFFX-ThrX-M_at 5.05283 A 0.559359

Total number of rows: 54675

Table truncated, full table size 1634 Kbytes.




Supplementary file Size Download File type/resource
GSM372721.CEL.gz 4.5 Mb (ftp)(http) CEL
GSM372721.CHP.gz 301.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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