|
| Status |
Public on May 20, 2009 |
| Title |
Healthy_CD4_3 |
| Sample type |
RNA |
| |
|
| Source name |
CD4 T cells
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age: 47
t_cell_purity: 92%
fab_type: Not applicable
prognostic_group: Not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
T cells were isolated by immunomagnetic selection. RNA was then extracted using Trizol.
|
| Label |
biotin
|
| Label protocol |
Affymetrix Expression GeneChip Protocol one-cycle procedure. Between 2 and 5micrograms of RNA was used as starting material.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 micrograms of cRNA were hybridised for 16 hours at 45C on Affymetrix U133Plus2 GeneChips. Chips were then washed and stained with SAPE in an Affymetrix GeneChip 450 Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Ayymetrix GeneChip 3000 scanner
|
| Description |
Healthy3 CD4
|
| Data processing |
Data were analysed with MicroArray Suite 5.0 (MAS 5.0) using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Feb 20, 2009 |
| Last update date |
Feb 23, 2009 |
| Contact name |
Rifca Le Dieu |
| E-mail(s) |
rledieu@gmail.com
|
| Organization name |
Queen Mary University of London
|
| Department |
Institute of Cancer
|
| Lab |
Centre for Medical Oncology
|
| Street address |
3rd Floor, John Vane Science Building, Charterhouse Square
|
| City |
London |
| ZIP/Postal code |
EC1M 6BQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14924 |
Characterisation of gene expression changes in T cells from patients presenting with AML compared with healthy T cells |
|
