|
| Status |
Public on Feb 01, 2020 |
| Title |
SAS-3.4/GFP_1 |
| Sample type |
RNA |
| |
|
| Source name |
GFP overexpressed SAS-3.4 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SAS-3.4
cell type: oral squamous cancer cell line
genotype/variation: GFP overexpression
molecule subtype: miRNA
|
| Treatment protocol |
The gene was overexpressed in SAS-3.4 cells and cultured for 48 hours. After 48 hours, total RNA of SAS cells was extracted.
|
| Growth protocol |
SAS cells were obtained from the Japanese Collection of Research Bioresources Cell Bank. SAS cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Invitrogen, CA, USA) supplemented with 10% heat-inactivated FBS, 1% AA and 10mg/mL Puromicine at 37 °C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using QIAzol and the miRNeasy Mini Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocols. RNA was quantified using a NanoDrop-1000 spectrophotometer and a Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, Calif.).
|
| Label |
Cy3
|
| Label protocol |
The miRNA Complete Labeling and Hyb Kit (Agilent Technologies)
|
| |
|
| Hybridization protocol |
Agilent one-color gene expression hyb/wash protocol
|
| Scan protocol |
Agilent SureScan G4900DA
|
| Description |
SAMPLE4
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 11.5.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
May 14, 2019 |
| Last update date |
Feb 02, 2020 |
| Contact name |
Yusuke Yamamoto |
| E-mail(s) |
yuyamamo@ncc.go.jp
|
| Phone |
+81-335422511
|
| Organization name |
National cancer center research institute
|
| Lab |
Cellular signaling
|
| Street address |
5-1-1, Tsukiji, Chuo-ku
|
| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16770 |
| Series (1) |
| GSE131166 |
CD44s regulated miRNAs in head and neck cancer cells. |
|
