|
Status |
Public on May 29, 2019 |
Title |
Control retina |
Sample type |
RNA |
|
|
Source name |
Control
|
Organism |
Mus musculus |
Characteristics |
tissue: Retina strain: C57BL/6J
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extraction was performed using the miRNeasy Micro kit (QIAGEN) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-ND1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent).
|
Label |
Cy3
|
Label protocol |
Cy3 labeled RNA was prepared from 100 ng RNA using the miRNA Complete Labeling and Hyb Kit (Agilent) according to the manufacturer's instructions.
|
|
|
Hybridization protocol |
Cy3-labelled RNA were hybridized Expression Hybridization kit (Agilent) for 22 hour at 55℃ following the manufacturers instructions.
|
Scan protocol |
After washed, the microarray were scanned by DNA Microarray Scanner (Agilent)
|
Data processing |
The scanning data was digitized using Feature Extraction ver 10.7.1.1 software (Agilent).
|
|
|
Submission date |
May 28, 2019 |
Last update date |
May 29, 2019 |
Contact name |
Yasuhisa Tanaka |
Phone |
743794525
|
Organization name |
Santen Pharmaceutical CO., LTD
|
Street address |
8916-16
|
City |
Takayama-cho Ikoma-shi, Nara |
State/province |
Japan |
ZIP/Postal code |
630-0101 |
Country |
Japan |
|
|
Platform ID |
GPL21265 |
Series (1) |
GSE131831 |
Tissue specific comprehensive gene analysis in a murine lens-induced myopia model |
|