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| Status |
Public on May 29, 2019 |
| Title |
Tissue specific comprehensive gene analysis in a murine lens-induced myopia model |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
To identify to identify target tissues and molecules involved with refractive myopic shift and axial length elongation in a murine lens-induced myopia model, we performed comprehensive analysis by microRNA array. Negative 30diptor (-30D) lens was fixed on right eye (-30D) of C57BL/6J mice (3weeks old, N=3) for 3 weeks, the refraction and the axial length were measured using a refractometer and a SD-OCT system in all eyes. Eye balls were enucleated and separated to cornea, iris, lens, retina, choroid and sclera. Total RNA was extracted from individual ocular components. MicroRNA expression analysis was carried out using Agilent Mouse miRNA Microarray (8×60K) miRBase21.0 (Agilent). Expression ratio calculation and miRNA varying expression were extracted by GeneSpring GX 14.5 (Agilent). After 3weeks of lens fixing, a refractive change and an axial length elongation change were observed (Normal vs -30D: 0.95 ± 1.85D vs -18.42 ± 3.98D, 0.155 mm ± 0.015mm vs 0.273 ± 0.009 mm), respectively. MiRNA expression changes that induced only by -30D lens fixing was confirmed in each part of the eyeball. By expression ratio calculation and miRNA varying expression analysis, upregulated miRNA (56 in cornea, 13 in iris, 6 in lens, 0 in retina, 29 in choroid and 30 in sclera) and downregulated miRNA (7 in cornea, 28 in iris, 17 in lens, 9 in retina, 7 in choroid and 40 in sclera) were observed. Overlapping miRNAs were also found while each eye tissues. In this study, miRNA varying expression were observed in each ocular part of the murine lens-induced myopia model. These miRNAs dysregulation may be functionally involved with the refractive myopia shift and the axial length elongation.
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| Overall design |
Eye balls were enucleated and separated to cornea, iris, lens, retina, choroid and sclera. About Each eye tisses, varying miRNA were comfirmed in control group vs lens induced group (Corne control vs cornea lens induced).
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| Contributor(s) |
Tanaka Y |
| Citation(s) |
31344984 |
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| Submission date |
May 28, 2019 |
| Last update date |
Sep 11, 2019 |
| Contact name |
Yasuhisa Tanaka |
| Phone |
743794525
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| Organization name |
Santen Pharmaceutical CO., LTD
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| Street address |
8916-16
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| City |
Takayama-cho Ikoma-shi, Nara |
| State/province |
Japan |
| ZIP/Postal code |
630-0101 |
| Country |
Japan |
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| Platforms (1) |
| GPL21265 |
Agilent-070155 Mouse miRNA Microarray (miRBase Release 21.0, miRNA ID version) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA545088 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE131831_RAW.tar |
72.4 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE131831_normalized.txt.gz |
66.2 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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