|
| Status |
Public on May 29, 2019 |
| Title |
0D iris |
| Sample type |
RNA |
| |
|
| Source name |
0D lens
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Iris
strain: C57BL/6J
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using the miRNeasy Micro kit (QIAGEN) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-ND1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent).
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled RNA was prepared from 100 ng RNA using the miRNA Complete Labeling and Hyb Kit (Agilent) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Cy3-labelled RNA were hybridized Expression Hybridization kit (Agilent) for 22 hour at 55℃ following the manufacturers instructions.
|
| Scan protocol |
After washed, the microarray were scanned by DNA Microarray Scanner (Agilent)
|
| Data processing |
The scanning data was digitized using Feature Extraction ver 10.7.1.1 software (Agilent).
|
| |
|
| Submission date |
May 28, 2019 |
| Last update date |
May 29, 2019 |
| Contact name |
Yasuhisa Tanaka |
| Phone |
743794525
|
| Organization name |
Santen Pharmaceutical CO., LTD
|
| Street address |
8916-16
|
| City |
Takayama-cho Ikoma-shi, Nara |
| State/province |
Japan |
| ZIP/Postal code |
630-0101 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21265 |
| Series (1) |
| GSE131831 |
Tissue specific comprehensive gene analysis in a murine lens-induced myopia model |
|
