|
Status |
Public on Apr 03, 2009 |
Title |
WT ProB, biological rep2 |
Sample type |
RNA |
|
|
Source name |
proB from bone marrow
|
Organism |
Mus musculus |
Characteristics |
cell type: proB genotype: wt
|
Treatment protocol |
For proB cell isolation, wt bone marrow cells were depleted with antibodies against Ter119, Mac-1, Gr-1, IgM, CD3, CD8a, TCRbeta, TCRgamma-delta, DX5 and remaining cells with a c-Kitlo CD19+ phenotype were sorted.
|
Growth protocol |
mice were bred and maintained under specific pathogen free condition in the animal facility.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array.
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
Description |
Gene expression data from mouse hematopoietic cells. proB cells
|
Data processing |
The data were were normalized by robust multi-chip analysis (RMA) (Bioconductor release 1.9) using PM-only models.
|
|
|
Submission date |
Mar 21, 2009 |
Last update date |
Mar 22, 2009 |
Contact name |
Jiangwen Zhang |
E-mail(s) |
jzhang@cgr.harvard.edu
|
Organization name |
Harvard University
|
Department |
FAS Center for Systems Biology
|
Street address |
7 Divinity Ave
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02138 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE15330 |
Gene expression data from mouse hematopoietic cells, Ikaros wt and null mutant |
|