|
| Status |
Public on Apr 03, 2009 |
| Title |
KO LSK GFPneg-lo, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse hematopoietic progenitors LSK cells with Ikaros-based GFP reporter that allows a clean separation of functionally distinct progenitor subsets
|
| Organism |
Mus musculus |
| Characteristics |
cell type: hematopoietic LSK GFPneg-lo (HSC)
genotype: KO
protocol: Ikaros-based GFP reporter with negative to very low level expression (GFPneg-lo) of GFP
|
| Treatment protocol |
LSK GFPneg-lo (HSC) were isolated from KO B-p-GFP-C mice
|
| Growth protocol |
The transgenic mice (B-p-GFP-C line, C57BL/6 x C3H)(Kaufmann et al., 2003) and Ikaros null mice (I74 line, C57BL/6 x 129SV) (Wang et al., 1996) were bred and maintained under specific pathogen free condition in the animal facility.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from mouse hematopoietic cells.
mouse hematopoietic LSK GFPneg-lo (HSC) from KO mouse
|
| Data processing |
The data were were normalized by robust multi-chip analysis (RMA) (Bioconductor release 1.9) using PM-only models.
|
| |
|
| Submission date |
Mar 21, 2009 |
| Last update date |
Mar 22, 2009 |
| Contact name |
Jiangwen Zhang |
| E-mail(s) |
jzhang@cgr.harvard.edu
|
| Organization name |
Harvard University
|
| Department |
FAS Center for Systems Biology
|
| Street address |
7 Divinity Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE15330 |
Gene expression data from mouse hematopoietic cells, Ikaros wt and null mutant |
|
