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Status |
Public on Apr 03, 2009 |
Title |
KO LK GFPhi, biological rep3 |
Sample type |
RNA |
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|
Source name |
mouse hematopoietic LK cells with Ikaros-based GFP reporter that allows a clean separation of functionally distinct progenitor subsets
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Organism |
Mus musculus |
Characteristics |
cell type: hematopoietic LK GFPhi(GMP) genotype: KO protocol: Ikaros-based GFP reporter with high level expression (GFPhi) of GFP
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Treatment protocol |
LK GFPhi (GMP) were isolated from KO B-p-GFP-C mice
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Growth protocol |
The transgenic mice (B-p-GFP-C line, C57BL/6 x C3H)(Kaufmann et al., 2003) and Ikaros null mice (I74 line, C57BL/6 x 129SV) (Wang et al., 1996) were bred and maintained under specific pathogen free condition in the animal facility.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array.
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Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
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Description |
Gene expression data from mouse hematopoietic cells. mouse hematopoietic LK GFPhi(GMP) from KO mouse
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Data processing |
The data were were normalized by robust multi-chip analysis (RMA) (Bioconductor release 1.9) using PM-only models.
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Submission date |
Mar 21, 2009 |
Last update date |
Mar 22, 2009 |
Contact name |
Jiangwen Zhang |
E-mail(s) |
jzhang@cgr.harvard.edu
|
Organization name |
Harvard University
|
Department |
FAS Center for Systems Biology
|
Street address |
7 Divinity Ave
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02138 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE15330 |
Gene expression data from mouse hematopoietic cells, Ikaros wt and null mutant |
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