|
| Status |
Public on Mar 01, 2010 |
| Title |
1ng/mL R4 DMSO day 5 Danio rerio 39 embryo |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
1ng/mL R4 DMSO day 5
|
| Organism |
Danio rerio |
| Characteristics |
developmental stage: day 5 embryo
treatment: 1ng/mL R4 DMSO
|
| Treatment protocol |
Embryos were dosed for 1 hour (from 4 to 5 hours post-fertilization) with 1 ng/mL TCDD in glass scintillation vials, with shaking.
|
| Growth protocol |
Embryos were incubated in 5 mL danieau in glass scintillation vials, with 15-20 embryos/vial.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen's RNEasy kit, according to the manufacturer's protocol.
|
| Label |
Cy5
|
| Label protocol |
Total RNA samples were labeled using the Low Input Linear Amplification Kit from Agilent Technologies along with Cy-linked dNTPs from Amersham Biosciences.
|
| |
|
| Channel 2 |
| Source name |
Zebrafish Fibroblast cell line Reference RNA
|
| Organism |
Danio rerio |
| Characteristics |
tissue: fibroblast cell line
|
| Treatment protocol |
Embryos were dosed for 1 hour (from 4 to 5 hours post-fertilization) with 1 ng/mL TCDD in glass scintillation vials, with shaking.
|
| Growth protocol |
Embryos were incubated in 5 mL danieau in glass scintillation vials, with 15-20 embryos/vial.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen's RNEasy kit, according to the manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
Total RNA samples were labeled using the Low Input Linear Amplification Kit from Agilent Technologies along with Cy-linked dNTPs from Amersham Biosciences.
|
| |
|
| |
| Hybridization protocol |
Hybridizations were conducted according to Agilent recommendations for 22K commercial microarrays.
|
| Scan protocol |
Arrays were scanned using the Agilent DNA Microarray scanner using factory settings.
|
| Description |
1ng/mL R4 DMSO day 5 Danio rerio 39 embryo
|
| Data processing |
Array images were processed using Agilent Feature Extraction Software v8.1.1
|
| |
|
| Submission date |
Mar 26, 2009 |
| Last update date |
Dec 22, 2009 |
| Contact name |
Patrick Hurban |
| E-mail(s) |
phurban@cogenics.com
|
| Phone |
919-379-5480
|
| Fax |
919-468-8766
|
| URL |
http://www.cogenics.com
|
| Organization name |
Cogenics, Inc.
|
| Department |
Scientific Affairs
|
| Street address |
100 Perimeter Park Dr Ste C
|
| City |
Morrisville |
| State/province |
NC |
| ZIP/Postal code |
27560 |
| Country |
USA |
| |
|
| Platform ID |
GPL2878 |
| Series (1) |
| GSE15410 |
AB* zebrafish embryos exposed to 1 ng/mL TCDD from 1d to 5d post-fertilization. |
|
