|
| Status |
Public on Jun 13, 2019 |
| Title |
MAL_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
oocyte
|
| Organism |
Sus scrofa |
| Characteristics |
developmental stage: MII
treatment: MAL
cell type: oocyte
|
| Treatment protocol |
MAL treatment was to dilute MAL (original concentration 0.75 M) dissolved in DMSO in IVM medium to give a final concentration of 1 mM.
|
| Growth protocol |
Porcine ovaries were obtained from a slaughterhouse and transported to the laboratory in 2 hr. Follicular fluid from 3–6 mm antral follicles were aspirated with an 19-gauge syringe. Cumulus oocyte complexes (COCs) with uniform cytoplasm and several layers
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
The RNA-seq libraries were generated following the Smart-seq2 protocol with minor modification (Picelli et al., 2014).
Libraries were constructed with TruSeq DNA library preparation kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Reads of RNA-seq were mapped to the Sus scrofa genome (sus11.1) with STAR (v2.5.3a)
Gene differential expression analysis using DESeq2 (v1.20.0) based on the reads count file obtained by HTSeq (v0.9.1)
Genome_build: sus11.1
Supplementary_files_format_and_content: Tab-delimited text files include read counts for each gene
|
| |
|
| Submission date |
Jun 12, 2019 |
| Last update date |
Jun 13, 2019 |
| Contact name |
Jing-Jing Zhang |
| E-mail(s) |
jjzhang@webmail.hzau.edu.cn
|
| Organization name |
Huazhong Agricultural University
|
| Department |
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction
|
| Lab |
Miaolab
|
| Street address |
1 Lion Rock Street
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL22918 |
| Series (1) |
| GSE132618 |
Melatonin protects against defects induced by malathion during porcine oocyte maturation |
|
| Relations |
| BioSample |
SAMN12041353 |
| SRA |
SRX6052632 |
