|
| Status |
Public on Feb 01, 2021 |
| Title |
kidney_diabets_rep1 [C9; re-analysis] |
| Sample type |
RNA |
| |
|
| Source name |
diabetic, 17 weeks
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain background: Sprague Dawley
age: 17 week
tissue: kidney
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total tissue RNA was extracted using total RNA Isolation Kit (mirVanaTM, ABI).
|
| Label |
biotin
|
| Label protocol |
biotin
|
| |
|
| Hybridization protocol |
The sample preparation and hybridation of each cDNAs on Affymetrix GeneChip® Mouse Gene 2.0 ST arrays based on manufacturer's instruction.
|
| Scan protocol |
Arrays were scanned using the GeneChip® Scanner 3000 Affymetrix)
|
| Description |
gene expression data from kidney of diabetic rat
|
| Data processing |
All arrays were uploaded into Genespring v12.5 (Agilent Tchnologies, USA) for data normalization, quality control and first-pass analysis. Differentially expressed genes were then identified through fold change as well as P value calculated with t-test. The threshold set for differentially regulated genes was a fold change>1.5 and a P value<0.05.
|
| |
|
| Submission date |
Jul 10, 2019 |
| Last update date |
Feb 02, 2021 |
| Contact name |
Qian Zhang |
| Organization name |
Peking Union Medical College Hospital
|
| Street address |
No. 1 Shuaifuyuan, Dongcheng District
|
| City |
Beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE134072 |
Gene expression alternations in Qishen Yiqi Dripping Pill-treated rats kidney |
|
| Relations |
| Reanalysis of |
GSM3935925 |
