|
| Status |
Public on Apr 01, 2010 |
| Title |
LECs from healthy tissue, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
LECs from healthy mammary skin of balb-c/nude mice
|
| Organism |
Mus musculus |
| Characteristics |
cell type: normal LECs
|
| Treatment protocol |
Tissue sections of healthy skin and tumors were briefly immunostained for the LEC marker LYVE-1 before isolating LECs by means of Laser Capture microdissection
|
| Growth protocol |
MDA-MB-435 xenografts were injected subcutaneously and were excised 8 weeks after injection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Plus Micro
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the FL-Ovation cDNA Biotin Module V2 from Nugen from 3 ug amplified cDNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 3 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G with autoloader.
|
| Description |
mRNA was subjected to 1 round of Ribo-Spia amplification prior to microarray analysis
|
| Data processing |
The data were analyzed with robust multi-array average (RMA) statistics using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Apr 21, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
milica Nikolic |
| E-mail(s) |
milica.nikolic@pharma.ethz.ch
|
| Phone |
0446333412
|
| Organization name |
ETH
|
| Department |
DCHAB
|
| Lab |
Detmar
|
| Street address |
Wolfgang-Pauli-Str.10
|
| City |
Zurich |
| ZIP/Postal code |
8093 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE15760 |
Comparative transcriptional profiling of tumor-associated and normal lymphatic endothelial cells |
|
| Relations |
| Reanalyzed by |
GSE119085 |
