Cells were plated 24 hours before treatment with 0.1% DMSO, 1 µM GSK3484862, then grown for 96 hours before collection.
Growth protocol
Cell lines were obtained from the American Type Culture Collection, the Deutsche Sammlung von Mikroorganismen und Zellbulturen, or AddexBio and maintained in the recommended cell culture media at 37°C in 5% CO2.
Extracted molecule
total RNA
Extraction protocol
RNA was isolated, labeled, and hybridized to Affymetrix GeneChip® Human Genome U133 Plus 2.0 arrays at Q2 Solutions.
Label
biotin
Label protocol
Not provided
Hybridization protocol
Not provided
Scan protocol
Not provided
Data processing
Differential gene expression analysis was performed using limma with default settings for priors and other parameters on human genome U133 Plus 2.0 Affymetrix microarray .CEL files. Expression changes with adjusted p-values < 0.05 and absolute log2 fold change > 1 were considered significant.
