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Status |
Public on Aug 08, 2022 |
Title |
Human_TRPS1R908Q_90days_rep2 |
Sample type |
genomic |
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Source name |
MCF-10A cell, TRPS1R908Q, 90days
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Organism |
Homo sapiens |
Characteristics |
cell type: Normal breast epithelial cells cell line: MCF-10A overexpression: TRPS1R908Q time: 90 days
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Growth protocol |
Cell lines were grown according to standard conditions.
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Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted from MCF-10A cell preparations using the QIAquick PCR Purification Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, PicoGreen and agarose gel electrophoresis.
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Label |
biotin
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Label protocol |
As per manufacturer (Affymetrix)
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Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
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Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 7G.
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Description |
Hybridized to SNP_6 sample name: 11A_TP1R908Q_rep2
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Data processing |
The array image was acquired using Affymetrix GeneChip® Operating Software (GCOS version 1.4). Copy number values were extracted and converted from CEL files to signal intensities using the Copy Number Variation Analysis module of Affymetrix® Genotyping Console 4.2 software. Processed data file includes: Actual nucleotide indicating the genotype of each SNP; Signal A = signal with first nucleotide; Signal B = signal with second nucleotide.
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Submission date |
Aug 08, 2019 |
Last update date |
Aug 08, 2022 |
Contact name |
Yu Zhang |
Organization name |
Peking University Health Science Center
|
Street address |
Xueyuan Road
|
City |
Beijing |
ZIP/Postal code |
100191 |
Country |
China |
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Platform ID |
GPL6801 |
Series (1) |
GSE135598 |
TRPS1 drives heterochromatic origin refiring and cancer genome evolution [copy number] |
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