|
| Status |
Public on May 19, 2009 |
| Title |
HL-60 + 0.5 % DMSO, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
HL-60 cells treated for 24h with 0.5 % DMSO as control
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HL-60 promyelocytic leukemia cell line
leukemia type by fab: M2
agent: control
|
| Treatment protocol |
HL-60 cells were treated for 24h with the different substances in 5ml tissue flasks
|
| Growth protocol |
HL-60 cells were cultivated in RPMI medium with 10 % FCS, 1 % penecillin and 1 % streptavidin at 37°C and 5 % CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on human GeneChip U133 2A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Agilent GeneArray Scanner 2500A.
|
| Description |
n/a
|
| Data processing |
Cel files were processed using RMA (affy-package, R2.0)
|
| |
|
| Submission date |
May 19, 2009 |
| Last update date |
Nov 22, 2011 |
| Contact name |
Charlotte Esser |
| E-mail(s) |
schloepi@gmx.de
|
| Phone |
00492113389252
|
| URL |
http://www.iuf.uni-duesseldorf.de/
|
| Organization name |
IUF
|
| Department |
Immunologie
|
| Street address |
Aufm Hennekamp 50
|
| City |
Düsseldorf |
| State/province |
NRW |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE16160 |
Expression data of HL-60 leukemia cells after treatment with Curcumin and TCDD |
|
| Relations |
| Reanalyzed by |
GSM838429 |
