|
| Status |
Public on Jan 23, 2020 |
| Title |
O.WT 6M A+C: 6-m-o EpSC rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Epidermal stem cells from 6-m-o mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 6 months
tissue: epidermal stem cells
|
| Treatment protocol |
No treatment was applied to the animals
|
| Growth protocol |
This experiment has been done with animals.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Micro Kit (QIAGEN, Catalog No. 74004) was used according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was quantified using 6000 Nano Chips (Agilent Technologies, Santa Clara, CA) and used (2.3 mg/sample) to generated labeled cRNA probes according to the manufacturer's instructions (Affymetrix).
|
| |
|
| Hybridization protocol |
Mouse Gene 1.0 ST arrays (Affymetrix) under standard conditions
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Gene expression data from epidermal stem cells
|
| Data processing |
Upon microarray hybridization, the raw data was normalized with RMA using R (v.3.3.1).
|
| |
|
| Submission date |
Sep 10, 2019 |
| Last update date |
Jan 23, 2020 |
| Contact name |
L. Francisco Lorenzo-Martín |
| E-mail(s) |
luis.lorenzomartin@epfl.ch
|
| Organization name |
École Polytechnique Fédérale de Lausanne (EPFL)
|
| Lab |
Laboratory of Stem Cell Bioengineering
|
| Street address |
EPFL – SV – IBI – LSCB – Station 15
|
| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE137176 |
Time-driven molding of the epidermal stem cell transcriptome |
|
