|
| Status |
Public on Aug 23, 2020 |
| Title |
5600_65900_K1812G: Epidermis, Hdac3 control 5 |
| Sample type |
RNA |
| |
|
| Source name |
E18.5 epidermis
|
| Organism |
Mus musculus |
| Characteristics |
tissue: epidermis
age: E18.5
experiment: Hdac3
genotype: control
litter: Hdac3 litter 2
|
| Treatment protocol |
Dorsal skin was floated dermis side down on dispase solution and incubated at 37ºC until the epidermis could be easily removed. Epidermis was stored in Trizol following the manufacturer's instructions
|
| Growth protocol |
Pregnant dams were placed on 1g/kg doxycycline chow from gestational age E8.5 to E18.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed using the Qiagen RNeasy kit
|
| Label |
biotin
|
| Label protocol |
250ng of total RNA was converted to first-strand cDNA using reverse transcriptase primed by poly(T) and random oligomers that incorporated the T7 promoter sequence. Second-strand cDNA synthesis was followed by in vitro transcription with T7 RNA polymerase for linear amplification of each transcript, and the resulting cRNA was converted to cDNA, fragmented, assessed by Bioanalyzer, and biotinylated by terminal transferase end labeling.
|
| |
|
| Hybridization protocol |
Five and a half micrograms of labeled cDNA were added to Affymetrix hybridization cocktails, heated at 99ºC for 5 min and hybridized for 16 h at 45ºC.
|
| Scan protocol |
A GeneChip 3000 7G scanner was used to collect fluorescence signal.
|
| Data processing |
Cel files were RMA normalized in Transcriptome Analysis Console (TAC, v4.0.2, ThermoFisher)
|
| |
|
| Submission date |
Sep 10, 2019 |
| Last update date |
Aug 22, 2024 |
| Contact name |
Sarah E Millar |
| E-mail(s) |
sarah.millar@mssm.edu
|
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Department |
Developmental, Cell and Regenerative Biology
|
| Street address |
Icahn Building, Floor 13 Room 020C, 1425 Madison Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE137234 |
HDAC3 ensures stepwise epidermal stratification via NCoR/SMRT-reliant mechanisms independent of its histone deacetylase activity (HDAC3 NCoRSMRT microarray) |
| GSE137235 |
HDAC3 ensures stepwise epidermal stratification via NCoR/SMRT-reliant mechanisms independent of its histone deacetylase activity |
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