|
| Status |
Public on May 08, 2014 |
| Title |
Escherichia coli_30mM H202_Rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
LW12, 30mM H2O2, 30min
|
| Organism |
Escherichia coli |
| Characteristics |
strain: K-12
substrain: LW12
genotype: luxS mutant
|
| Treatment protocol |
Two conditions: 10mM hydrogen peroxide, 37 degree Celsius, 250rpm, 30min; 30mM hydrogen peroxide, 37 degree Celsius, 250rpm, 30min.
|
| Growth protocol |
In LB plus 0.8% glucose cells grew overnight, and then diluted to OD600=0.02, followed by culture until OD600 reaches 1.0. The culture was performed at 37 degree Celsius and 250rpm.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated after 30 min incubation with hydrogen peroxide using RNeasy Mini kit (Qiagen, Inc., Valencia, CA) according to the manufacturer’s protocol.
|
| Label |
Cy5
|
| Label protocol |
Approximately 10 µg of total RNA was processed to produce Cy5- or Cy3-labelled cDNA targets.
|
| |
|
| Channel 2 |
| Source name |
W3110, 30mM H2O2, 30min
|
| Organism |
Escherichia coli |
| Characteristics |
strain: K-12
substrain: W3110
genotype: wild type
|
| Treatment protocol |
Two conditions: 10mM hydrogen peroxide, 37 degree Celsius, 250rpm, 30min; 30mM hydrogen peroxide, 37 degree Celsius, 250rpm, 30min.
|
| Growth protocol |
In LB plus 0.8% glucose cells grew overnight, and then diluted to OD600=0.02, followed by culture until OD600 reaches 1.0. The culture was performed at 37 degree Celsius and 250rpm.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated after 30 min incubation with hydrogen peroxide using RNeasy Mini kit (Qiagen, Inc., Valencia, CA) according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
Approximately 10 µg of total RNA was processed to produce Cy5- or Cy3-labelled cDNA targets.
|
| |
|
| |
| Hybridization protocol |
Standard Agilent procedures.
|
| Scan protocol |
Scanned on Agilent Scanner G2505B US45103124, and images were quantified using Agilent Feature Extraction Software v. 9.5.3.1.
|
| Description |
Biological replicate 2 of 2.
|
| Data processing |
Agilent Feature Extraction Software v. 9.5.3.1 was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
May 28, 2009 |
| Last update date |
May 08, 2014 |
| Contact name |
Hua Ling |
| Organization name |
Nanyang Technological University
|
| Department |
Department of Chemical and Biomolecular Engineering, SCBE
|
| Lab |
Prof. Matthew Wook Chang
|
| Street address |
62 Nanyang Drive
|
| City |
Singapore |
| ZIP/Postal code |
637459 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL8609 |
| Series (1) |
| GSE16335 |
Escherichia coli K-12: luxS mutant LW12 vs. wild type W3110 under H2O2 stress |
|
