|
Status |
Public on Sep 30, 2022 |
Title |
S5B |
Sample type |
SRA |
|
|
Source name |
T4
|
Organism |
Homo sapiens |
Characteristics |
tissue: Tumor
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Description |
all.counts.exp.txt
|
Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13 HISAT2.1.0 mapping to reference genome count counting Genome_build: GRCh38 Supplementary_files_format_and_content: TXT file contain counts for each sample
|
|
|
Submission date |
Sep 30, 2019 |
Last update date |
Sep 30, 2022 |
Contact name |
Chi Zhou |
E-mail(s) |
zhouchi2@mail2.sysu.edu.cn
|
Organization name |
The Sixth Affiliated Hospital of Sun Yat-sen University
|
Street address |
26 Yuancun Erheng Rd
|
City |
Guangzhou |
ZIP/Postal code |
510000 |
Country |
China |
|
|
Platform ID |
GPL20795 |
Series (1) |
GSE138202 |
Next Generation Sequencing of CRC tissue |
|
Relations |
BioSample |
SAMN12875812 |
SRA |
SRX6926031 |