|
| Status |
Public on Sep 30, 2022 |
| Title |
S6A |
| Sample type |
SRA |
| |
|
| Source name |
N5
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Normal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
all.counts.exp.txt
|
| Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13
HISAT2.1.0 mapping to reference genome
count counting
Genome_build: GRCh38
Supplementary_files_format_and_content: TXT file contain counts for each sample
|
| |
|
| Submission date |
Sep 30, 2019 |
| Last update date |
Sep 30, 2022 |
| Contact name |
Chi Zhou |
| E-mail(s) |
zhouchi2@mail2.sysu.edu.cn
|
| Organization name |
The Sixth Affiliated Hospital of Sun Yat-sen University
|
| Street address |
26 Yuancun Erheng Rd
|
| City |
Guangzhou |
| ZIP/Postal code |
510000 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (1) |
| GSE138202 |
Next Generation Sequencing of CRC tissue |
|
| Relations |
| BioSample |
SAMN12875834 |
| SRA |
SRX6926041 |
