|
| Status |
Public on Apr 20, 2020 |
| Title |
J3T1shA |
| Sample type |
RNA |
| |
|
| Source name |
J3T1shA
|
| Organism |
Canis lupus familiaris |
| Characteristics |
tissue: glioma
subclone: J3T1shA
expression: knockdown of annexin A2
|
| Growth protocol |
Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum and 100U penicillin and 0.1mg ml-1 storeptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of J3T-1, J3T-2, J3T-1shA, and J3T2A were isolated using RNeasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were synthesized by GeneChip 3' IVT PLUS Reagent Kit from 250 ng total RNA according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45˚C on GeneChip Canine Genome 2.0 Array. Arrays were washed and stained in the GeneChip Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned using GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from J3T-1shA
|
| Data processing |
Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method.
|
| |
|
| Submission date |
Oct 03, 2019 |
| Last update date |
Apr 20, 2020 |
| Contact name |
Yuji Matsumoto |
| Organization name |
Okayama University
|
| Department |
Neurological Surgery
|
| Street address |
2-5-1 Shikata, kita-ku
|
| City |
Okayama |
| ZIP/Postal code |
7008558 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3738 |
| Series (1) |
| GSE138374 |
Expression data of J3T-1, J3T-2, J3T-1shA, J3T-2A glioma |
|
