|
| Status |
Public on Mar 09, 2021 |
| Title |
Acute myeloid leukemia cell line U937 |
| Sample type |
genomic |
| |
|
| Source name |
Acute myeloid leukemia cell line U937
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U937
cell type: Acute myeloid leukemia cell line
|
| Treatment protocol |
All cell lines were untreated prior to SNP analysis.
|
| Growth protocol |
KG-1 and U937 were grown in RPMI supplemented with 10 % fetal calf serum and 1 % penicillin/streptomycin. UCSD-AML1 and ELF-153 were grown in RPMI supplemented with 20 % fetal calf serum, 1 % penicillin/streptomycin and 5 ng/ml human GM-CSF.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the Quiagen DNeasy Blood and Tissue DNA extraction Kit according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Samples were labeled using the Affymetrix CytoScan Reagent and the SNP 6.0 Core Reagent Kits (USCD-AML1/ELF-153/KG-1 and U937, respectively)
|
| |
|
| Hybridization protocol |
according to manufacturer (Affymetrix)
|
| Scan protocol |
according to manufacturer (Affymetrix)
|
| Data processing |
CEL files were analysed using the Affymetrix Chromosome Analysis Suite ChAS (USCD-AMl1/ELF-153/KG-1) and the Affymetrix Genoyping Console Software (U937); data was annotated to the reference genome assembly GRCh37(hg19).
|
| |
|
| Submission date |
Oct 04, 2019 |
| Last update date |
Mar 09, 2021 |
| Contact name |
Gabriele Greve |
| E-mail(s) |
gabriele.greve@uniklinik-freiburg.de
|
| Organization name |
University of Freiburg Medical Center
|
| Department |
Department of Internal Medicine 1
|
| Lab |
Division of Hematology, Oncology and SCT, Lab Prof. Michael Lübbert
|
| Street address |
Hugstetter Str 55
|
| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE138438 |
Copy-number profiling by SNP array of 4 acute myeloid leukemia (AML) cell lines |
|
