|
| Status |
Public on Jun 02, 2009 |
| Title |
Prop_0h_A |
| Sample type |
RNA |
| |
|
| Source name |
propagation, 0h
|
| Organism |
Saccharomyces pastorianus |
| Characteristics |
strain: CB11
timepoint: 0 hours
|
| Treatment protocol |
yeast sampling: Triplicate samples of the lager yeast strain CB11 were sampled from a 140 hl (14,000 l) propagation vessel immediately after inoculation and at intervals throughout a 30-hour propagation period. The yeast batch was incubated aerobically in an 8 hl propagation vessel prior to incubation in the principal (140 hl) vessel. Propagation wort (80% malt and 20% maltose adjunct) supplement_protocoled to 0.5 mg l-1 Zn2+ (added as zinc sulphate heptahydrate) and was oxygenated with a ramped supply of molecular oxygen (5-100 l min-1) to give a constant dissolved oxygen concentration of 7-8 mg l-1. Temperature of the wort was maintained throughout at 20°C. Specific gravity of the wort was 17° Plato. Cells and wort were separated by centrifugation at 4°C. Cell pellets for RNA extraction were flash-frozen in liquid nitrogen and stored at -80°C until required.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was carried out following the method of Lyne et al. RNA yield and purity were determined using an Agilent 2100 Bioanalyser (Agilent Technologies Inc., Santa Clara, California).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from total RNA with Affymetrix GeneChip Labeling and Control Reagents.
|
| |
|
| Hybridization protocol |
Hybridization, washing, staining procedures were conducted with Affymetrix Fluidics Station as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
|
| Scan protocol |
Scanning done with GeneChip Scanner as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
|
| Description |
S. pastorianus 0hrs
|
| Data processing |
Four time points from the fermentation (0, 4, 8, and 30 hours after pitching) were chosen for microarray analysis. All analyses were carried out in triplicate, with each replicate processed separately. In total, therefore, 12 samples were used in this investigation. CEL files were generated using the GeneChip® operating system (GCOS version 5.0; Affymetrix). The data were analyzed with Affymetrix MAS5 software.
|
| |
|
| Submission date |
Jun 01, 2009 |
| Last update date |
Jun 01, 2009 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
affy@arabidopsis.info
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE16376 |
Differential yeast gene transcription during brewery propagation |
|
