|
| Status |
Public on Jun 02, 2009 |
| Title |
Prop_4h_C |
| Sample type |
RNA |
| |
|
| Source name |
propagation, 4h
|
| Organism |
Saccharomyces pastorianus |
| Characteristics |
strain: CB11
timepoint: 4 hours
|
| Treatment protocol |
yeast sampling: Triplicate samples of the lager yeast strain CB11 were sampled from a 140 hl (14,000 l) propagation vessel immediately after inoculation and at intervals throughout a 30-hour propagation period. The yeast batch was incubated aerobically in an 8 hl propagation vessel prior to incubation in the principal (140 hl) vessel. Propagation wort (80% malt and 20% maltose adjunct) supplement_protocoled to 0.5 mg l-1 Zn2+ (added as zinc sulphate heptahydrate) and was oxygenated with a ramped supply of molecular oxygen (5-100 l min-1) to give a constant dissolved oxygen concentration of 7-8 mg l-1. Temperature of the wort was maintained throughout at 20°C. Specific gravity of the wort was 17° Plato. Cells and wort were separated by centrifugation at 4°C. Cell pellets for RNA extraction were flash-frozen in liquid nitrogen and stored at -80°C until required.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was carried out following the method of Lyne et al. RNA yield and purity were determined using an Agilent 2100 Bioanalyser (Agilent Technologies Inc., Santa Clara, California).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from total RNA with Affymetrix GeneChip Labeling and Control Reagents.
|
| |
|
| Hybridization protocol |
Hybridization, washing, staining procedures were conducted with Affymetrix Fluidics Station as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
|
| Scan protocol |
Scanning done with GeneChip Scanner as described in the Affymetrix GeneChip Expression Analysis Technical Manual.
|
| Description |
S. pastorianus 4hrs
|
| Data processing |
CEL files were generated using the GeneChip® operating system (GCOS version 5.0; Affymetrix). The data were analyzed with Affymetrix MAS5 software.
|
| |
|
| Submission date |
Jun 01, 2009 |
| Last update date |
Jun 01, 2009 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
affy@arabidopsis.info
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE16376 |
Differential yeast gene transcription during brewery propagation |
|
