|
| Status |
Public on Dec 21, 2023 |
| Title |
RGI5_DMSO4 |
| Sample type |
SRA |
| |
|
| Source name |
RiD-RGI5/BAK1
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
genotype: RiD-RGI5/BAK1
tissue: root from 7-day-old-germination
agent: mock
|
| Treatment protocol |
Each plant was transferred on a filter paper containing 1/2 MS solution with mock, 100 nM rapamycin (RiD-RGI5/BAK1) or 1 µM rapamycin (RiD-RGI1/BAK1) and incubated for 4 hours with gentle shaking in light at 23°C. WT plants were treated with 100 nM RGF1 peptide for 4 hours.
|
| Growth protocol |
Seedlings were grown for 7 days after germination.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA from each samples was isolated using an RNeasy Plant Mini Kit (Qiagen, Japan)
The isolated total RNA was processed for preparing mRNA sequencing library using the Illumina TruSeq Stranded mRNA Sample preparation kit (Illumina) accoding to the manufacturer’s protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
mock
|
| Data processing |
The quantification of transcript expression was performed using Salmon
Differential expression analysis was performed using DESeq2
Genome_build: Araport11
Supplementary_files_format_and_content: abundance measurements
|
| |
|
| Submission date |
Oct 09, 2019 |
| Last update date |
Dec 21, 2023 |
| Contact name |
Jungmook Kim |
| E-mail(s) |
jungmkim@jnu.ac.kr
|
| Phone |
+82-62-5305187
|
| Organization name |
Chonnam national university
|
| Department |
Bioenergy science and technology
|
| Lab |
Plant cell signaling and Biomass control
|
| Street address |
77, yongbong-ro
|
| City |
Gwangju |
| ZIP/Postal code |
61186 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL26208 |
| Series (1) |
| GSE138627 |
Control of lateral root formation by rapamycin-induced dimerization of engineered RGI/BAK1 and by BIR3 chimera |
|
| Relations |
| BioSample |
SAMN12999712 |
| SRA |
SRX6968799 |
