|
| Status |
Public on Oct 23, 2019 |
| Title |
Non-malignant lung 05L34_N |
| Sample type |
genomic |
| |
|
| Source name |
Non-malignant lung
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Non-malignant lung
patient: 05L34
sample: 05L34_N
tissue: Normal
age (years): 73
Sex: Female
race: Asian
smoking history: Never
pack-years: 0
Stage: NA
egfr mutation: NA
kras mutation: NA
|
| Growth protocol |
Fresh frozen tissue
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted using standard phenol:chloroform procedures
|
| Label |
biotin
|
| Label protocol |
DNA was labeled according to manufacturer's instructions
|
| |
|
| Hybridization protocol |
DNA was hybridized according to manufacturer's instructions
|
| Scan protocol |
Arrays were scanned according to manufacturer's instructions
|
| Description |
Fresh frozen non-malignant lung sample
|
| Data processing |
Probe intensity files were processed in Partek Genomics Suite (Partek Incorporated, Missouri, USA). Probe sequencing, GC content, fragment length, and background adjustments were applied to correct for biases in signal intensities. DNA copy number profiles were generated in the Copy Number, Paired Analysis Workflow using patient-matched non-malignant lung as a baseline for each lung tumour sample. Segmentation was applied using stringent significance thresholds to identify regions of copy number gain and loss (signal to noise >0.3, minimum of 50 markers per segment, p-value < 10-7). Segments were merged using a 1 Mbp windown. Genomic mapping was based on the hg18 genomic build.
|
| |
|
| Submission date |
Oct 23, 2019 |
| Last update date |
Oct 23, 2019 |
| Contact name |
Katey SS Enfield |
| E-mail(s) |
kenfield@bccrc.ca, katey.enfield@crick.ac.uk
|
| Phone |
6046758111
|
| Organization name |
BC Cancer Research Centre
|
| Street address |
675 West 10th Ave
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6801 |
| Series (2) |
| GSE137481 |
Epithelial Tumour Suppressor ELF3 is a Lineage-Specific Amplified Oncogene in Lung Adenocarcinoma |
| GSE139294 |
DNA copy number profiling of lung adenocarcinomas and patient-matched non-malignant lung |
|
