|
| Status |
Public on Jan 01, 2011 |
| Title |
Whole-genome transcriptional profile in Streptococcus mutans strain UA159 (sample #1) |
| Sample type |
RNA |
| |
|
| Source name |
Streptococcus mutans strain UA159
|
| Organism |
Streptococcus mutans |
| Characteristics |
genotype: wildtype
|
| Growth protocol |
The Streptococcus mutans was grown in Todd-Hewitt medium (BBL; Becton Dickinson) supplemented with 0.2% yeast extract (THY)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed as described by Biswas et al. 2007. Regulation of gbpC expression in Streptococcus mutans. J. Bacteriol. 189:6521-6531
|
| Label |
Cy3
|
| Label protocol |
The probe was labeled according to the NimbleGen Arrays User's Guide
|
| |
|
| Hybridization protocol |
Hybridization protocol was done according to the NimbleGen Arrays User's Guide
|
| Scan protocol |
Scan protocol was done according to the NimbleGen Arrays User's Guide
|
| Description |
The human pathogen Streptococcus mutans is a gram-positive bacterium that residues in the oral cavity and is considered to be the primary etiological agent in the formation of dental caries
The whole procedure of RNA labelling, hybridization and detection has been previously published in NimbleGen Arrays User's Guide
|
| Data processing |
Data processing was done according to the NimbleGen Arrays User's Guide
|
| |
|
| Submission date |
Jun 08, 2009 |
| Last update date |
Jan 01, 2011 |
| Contact name |
Alexander V Dmitriev |
| E-mail(s) |
admitriev10@yandex.ru
|
| Organization name |
Kansas University Medical Center
|
| Street address |
3901 Rainbow Boulevard
|
| City |
Kansas City |
| State/province |
KS |
| ZIP/Postal code |
66160 |
| Country |
USA |
| |
|
| Platform ID |
GPL8671 |
| Series (1) |
| GSE16500 |
CovR-regulated genes in Streptococcus mutans strain UA159 |
|
