|
| Status |
Public on Jan 01, 2011 |
| Title |
Whole-genome transcriptional profile in Streptococcus mutans strain UA159 (covR mutant, sample #1) |
| Sample type |
RNA |
| |
|
| Source name |
Streptococcus mutans strain UA159 (covR mutant)
|
| Organism |
Streptococcus mutans |
| Characteristics |
genotype: covR mutant
|
| Growth protocol |
The Streptococcus mutans was grown in Todd-Hewitt medium (BBL, Becton Dickinson) supplemented with 0.2% yeast extract (THY)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed as described by Biswas et al. 2007. Regulation of gbpC expression in Streptococcus mutans. J. Bacteriol. 189:6521-6531
|
| Label |
Cy3
|
| Label protocol |
The probe was labeled according to the NimbleGen Arrays User's Guide
|
| |
|
| Hybridization protocol |
Hybridization protocol was done according to the NimbleGen Arrays User's Guide
|
| Scan protocol |
Scan protocol was done according to the NimbleGen Arrays User's Guide
|
| Description |
covR mutant of Streptococcus mutans strain UA159
The whole procedure of RNA labeling, hybridization and detection has been previously published in NimbleGen Arrays User’s Guide
|
| Data processing |
Data processing was done according to the NimbleGen Arrays User's Guide
|
| |
|
| Submission date |
Jun 08, 2009 |
| Last update date |
Jan 01, 2011 |
| Contact name |
Alexander V Dmitriev |
| E-mail(s) |
admitriev10@yandex.ru
|
| Organization name |
Kansas University Medical Center
|
| Street address |
3901 Rainbow Boulevard
|
| City |
Kansas City |
| State/province |
KS |
| ZIP/Postal code |
66160 |
| Country |
USA |
| |
|
| Platform ID |
GPL8671 |
| Series (1) |
| GSE16500 |
CovR-regulated genes in Streptococcus mutans strain UA159 |
|
