|
| Status |
Public on Jun 29, 2021 |
| Title |
A549 dnCRTC, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
A549 dnCRTC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
cell type: human lung carcinoma epithelial cells
genotype/variation: pMSCV retroviruses expressing dnCRTC [CRTC1(1-55aa)-nls-GFP]
|
| Treatment protocol |
GFP control and dnCRTC
|
| Growth protocol |
Cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% inactivated fetal bovine serum and penicillin/streptomycin (100U/ml) at 37°C under 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the Trizol Reagent (Invitrogen) and purified by the Qiagen Column with in column DNase digestion.
|
| Label |
biotin
|
| Label protocol |
Biotinylated sscDNA were prepared according to the standard Affymetrix protocol from 400 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, sscDNA were hybridized for 16 hr at 45C on Affymetrix Human Transcriptome Array 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner
|
| Description |
W24_dnCRTC
|
| Data processing |
The data were normalized using RMA normalization approach with Affymetrix Expression Console.
|
| |
|
| Submission date |
Nov 10, 2019 |
| Last update date |
Jun 29, 2021 |
| Contact name |
Lizi Wu |
| Organization name |
University of Florida
|
| Street address |
2033 Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (2) |
| GSE140188 |
Identification of the dnCRTC-regulated genes in lung cancer cells [microarray] |
| GSE157722 |
Identification of the dnCRTC-regulated genes in lung cancer cells |
|
